Document Detail

Fully phosphorylated fetuin-A forms a mineral complex in the serum of rats with adenine-induced renal failure.
MedLine Citation:
PMID:  19190677     Owner:  NLM     Status:  MEDLINE    
The serum glycoprotein fetuin-A is an important inhibitor of extra-osseous calcification, but correlations between serum fetuin-A levels and the extent of vascular calcification are controversial. In this study, we used a rat model of adenine-induced renal failure with secondary hyperparathyroidism that exhibits all characteristic features of patients with chronic kidney disease. These rats had medial vascular calcification along with reduced levels of both serum and hepatic fetuin-A. Treatment with an inhibitor of ectopic calcification, alendronate, decreased bone turnover and eliminated completely the vascular calcification in this rat model, but there was no change in the levels of hepatic and serum fetuin-A. Centrifugation of the serum of untreated rats with renal failure gave a small precipitate composed of fetuin-A, calcium, magnesium, and phosphate; this complex, absent from normal rat serum, was not found in the serum of alendronate-treated rats with renal failure. Rat serum contained three types of phosphorylated fetuin-A, as well as unphosphorylated forms, but only the fully phosphorylated fetuin-A was present in the mineral complex. The amount of this complex reflected the risk of mineral precipitation. Our results suggest that the measurement of serum fetuin-mineral complex rather than fetuin-A alone might provide a better indication of extra-osseous calcification propensity.
Isao Matsui; Takayuki Hamano; Satoshi Mikami; Naohiko Fujii; Yoshitsugu Takabatake; Yasuyuki Nagasawa; Noritaka Kawada; Takahito Ito; Hiromi Rakugi; Enyu Imai; Yoshitaka Isaka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-04
Journal Detail:
Title:  Kidney international     Volume:  75     ISSN:  1523-1755     ISO Abbreviation:  Kidney Int.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-15     Completed Date:  2009-07-07     Revised Date:  2010-11-29    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  915-28     Citation Subset:  IM    
Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, Osaka, Japan.
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MeSH Terms
Adenine / adverse effects
Alendronate / pharmacology
Calcinosis / drug therapy,  etiology*
Chemical Precipitation
Liver / metabolism
Minerals / blood,  metabolism*
Rats, Wistar
Renal Insufficiency / blood*,  chemically induced,  metabolism
alpha-Fetoproteins / metabolism*
Reg. No./Substance:
0/Minerals; 0/alpha-Fetoproteins; 66376-36-1/Alendronate; 73-24-5/Adenine
Comment In:
Kidney Int. 2010 Dec;78(11):1187; author reply 1187-9   [PMID:  21076451 ]
Kidney Int. 2009 May;75(9):874-6   [PMID:  19367310 ]
Kidney Int. 2009 Oct;76(8):915; author 915-6   [PMID:  19789551 ]

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