Document Detail


Fully integrated glass microfluidic device for performing high-efficiency capillary electrophoresis and electrospray ionization mass spectrometry.
MedLine Citation:
PMID:  18698800     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A microfabricated device has been developed in which electrospray ionization is performed directly from the corner of a rectangular glass microchip. The device allows highly efficient electrokinetically driven separations to be coupled directly to a mass spectrometer (MS) without the use of external pressure sources or the insertion of capillary spray tips. An electrokinetic-based hydraulic pump is integrated on the chip that directs eluting materials to the monolithically integrated spray tip. A positively charged surface coating, PolyE-323, is used to prevent surface interactions with peptides and proteins and to reverse the electroosmotic flow in the separation channel. The device has been used to perform microchip CE-MS analysis of peptides and proteins with efficiencies over 200,000 theoretical plates (1,000,000 plates/m). The sensitivity and stability of the microfabricated ESI source were found to be comparable to that of commercial pulled fused-silica capillary nanospray sources.
Authors:
J S Mellors; V Gorbounov; R S Ramsey; J M Ramsey
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-08-13
Journal Detail:
Title:  Analytical chemistry     Volume:  80     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-15     Completed Date:  2008-10-30     Revised Date:  2011-08-01    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6881-7     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of North Carolina at Chapel Hill, Chapman Hall, Room 251, Chapel Hill, North Carolina 27599-3216, USA.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Animals
Cattle
Electrophoresis, Capillary / instrumentation,  methods*
Glass / chemistry*
Microfluidic Analytical Techniques / instrumentation,  methods*
Peptides / isolation & purification
Proteins / isolation & purification
Proteomics
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization / instrumentation,  methods*
Surface Properties
Grant Support
ID/Acronym/Agency:
N01-HV-28182/HV/NHLBI NIH HHS; R33 CA083238-01/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Peptides; 0/Proteins
Comments/Corrections

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