Document Detail


Full-term development of hamster embryos produced by injection of round spermatids into oocytes.
MedLine Citation:
PMID:  14998912     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The golden hamster is a mammal in which microinjection of round spermatids into oocytes (ROSI) was first attempted. However, no live ROSI offspring have ever been obtained in this species. This is the first report of live hamster offspring obtained by round spermatid injection. Over 90% of oocytes, injected with round spermatids, were activated without any additional stimulation. The proportion of the oocytes that were fertilized normally and that developed to morulae and blastocysts was higher when the plasma membranes of the spermatids were broken before injection, as compared with when the membranes were left intact. Five percent of 57 ROSI morulae/blastocysts developed into live offspring after transfer to foster mothers.
Authors:
Kosuke Haigo; Yasuhiro Yamauchi; Futoshi Yazama; Ryuzo Yanagimachi; Toshitaka Horiuchi
Publication Detail:
Type:  Journal Article     Date:  2004-03-03
Journal Detail:
Title:  Biology of reproduction     Volume:  71     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-22     Completed Date:  2005-01-05     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  194-8     Citation Subset:  IM    
Affiliation:
Graduate School of Applied Biosciences, Hiroshima Prefectural University, Hiroshima, 727-0023 Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Cell Shape
Cricetinae / embryology*
Embryo Transfer
Embryonic Development
Female
Fertilization
Gestational Age
Male
Mesocricetus
Sperm Injections, Intracytoplasmic*
Spermatids / cytology*

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