| Full-term development of hamster embryos produced by injection of round spermatids into oocytes. | |
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MedLine Citation:
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PMID: 14998912 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The golden hamster is a mammal in which microinjection of round spermatids into oocytes (ROSI) was first attempted. However, no live ROSI offspring have ever been obtained in this species. This is the first report of live hamster offspring obtained by round spermatid injection. Over 90% of oocytes, injected with round spermatids, were activated without any additional stimulation. The proportion of the oocytes that were fertilized normally and that developed to morulae and blastocysts was higher when the plasma membranes of the spermatids were broken before injection, as compared with when the membranes were left intact. Five percent of 57 ROSI morulae/blastocysts developed into live offspring after transfer to foster mothers. |
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Authors:
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Kosuke Haigo; Yasuhiro Yamauchi; Futoshi Yazama; Ryuzo Yanagimachi; Toshitaka Horiuchi |
Publication Detail:
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Type: Journal Article Date: 2004-03-03 |
Journal Detail:
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Title: Biology of reproduction Volume: 71 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-06-22 Completed Date: 2005-01-05 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: United States |
Other Details:
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Languages: eng Pagination: 194-8 Citation Subset: IM |
Affiliation:
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Graduate School of Applied Biosciences, Hiroshima Prefectural University, Hiroshima, 727-0023 Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Cell Shape Cricetinae / embryology* Embryo Transfer Embryonic Development Female Fertilization Gestational Age Male Mesocricetus Sperm Injections, Intracytoplasmic* Spermatids / cytology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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