Document Detail


Full-length genome sequences of five hepatitis C virus isolates representing subtypes 3g, 3h, 3i and 3k, and a unique genotype 3 variant.
MedLine Citation:
PMID:  23152370     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We characterized the full-length genomes of five distinct hepatitis C virus (HCV)-3 isolates. These represent the first complete genomes for subtypes 3g and 3h, the second such genomes for 3k and 3i, and of one novel variant presently not assigned to a subtype. Each genome was determined from 18-25 overlapping fragments. They had lengths of 9579-9660 nt and each contained a single ORF encoding 3020-3025 aa. They were isolated from five patients residing in Canada; four were of Asian origin and one was of Somali origin. Phylogenetic analysis using 64 partial NS5B sequences differentiated 10 assigned subtypes, 3a-3i and 3k, and two additional lineages within genotype 3. From the data of this study, HCV-3 full-length sequences are now available for six of the assigned subtypes and one unassigned. Our findings should add insights to HCV evolutionary studies and clinical applications.
Authors:
Ling Lu; Chunhua Li; Jie Yuan; Teng Lu; Hiroaki Okamoto; Donald G Murphy
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-11-14
Journal Detail:
Title:  The Journal of general virology     Volume:  94     ISSN:  1465-2099     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-19     Completed Date:  2013-04-17     Revised Date:  2014-03-07    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  543-8     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/JF735121;  JF735122;  JF735123;  JF735124;  JF735125
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Biological Evolution
Gene Expression Regulation, Viral
Genome, Viral*
Genotype*
Hepacivirus / classification*,  genetics*
Hepatitis C / virology*
Humans
Molecular Sequence Data
Phylogeny
Grant Support
ID/Acronym/Agency:
R01 AI080734/AI/NIAID NIH HHS; R01 AI080734-03A/AI/NIAID NIH HHS
Comments/Corrections

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