| Fuel-induced insulin release in vitro from insulinomas transplanted into the rat kidney. | |
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MedLine Citation:
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PMID: 6140199 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We studied the release of insulin, glucagon, and somatostatin in response to glucose, glyceraldehyde (GA), and alpha-ketoisocaproate (KIC) from rat kidneys containing transplanted insulinomas. Kidneys were perfused about 11 wk after transplantation when the plasma glucose concentration of the fed animals had decreased from 180 +/- 7 to 95.1 +/- 9.9 mg/dl and plasma insulin concentrations had increased from 2.6 +/- 0.5 to 14.2 +/- 2.0 ng/ml. The insulin content of the tumor-containing kidney ranged from 40 to 679 micrograms; the glucagon and somatostatin concentrations ranged from undetectable levels to 3.7 micrograms and 248 ng, respectively. The average response to 30 mM glucose and 10 mM GA was a four- to fivefold increase in insulin secretion, whereas 30 mM KIC caused a 16- to 28-fold increase. In vitro stimulation of the insulinoma with 30 mM glucose primed the beta-cell response to a second stimulus following a short rest period. Cytochalasin B did not enhance this primed glucose response. Diazoxide inhibited glucose, GA, and KIC-stimulated insulin release. Glucose, GA, and KIC stimulated glucagon release in 2 of 17 insulinomas studied here. Somatostatin release was not seen in any of the experiments. These findings show that this islet cell tumor transplanted under the kidney capsule releases insulin in response to physiologic and model fuel substances. Thus, this particular transplantable tumor offers an opportunity to study the biochemistry and biophysics that underlie fuel-stimulated insulin release. |
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Authors:
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M Hoenig; D C Ferguson; F M Matschinsky |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Diabetes Volume: 33 ISSN: 0012-1797 ISO Abbreviation: Diabetes Publication Date: 1984 Jan |
Date Detail:
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Created Date: 1984-02-21 Completed Date: 1984-02-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372763 Medline TA: Diabetes Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1-7 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenoma, Islet Cell
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secretion* Animals Cytochalasin B / pharmacology Diazoxide / pharmacology Glucagon / secretion Glucose / pharmacology* Glyceraldehyde / pharmacology* Humans Insulin / secretion* Insulinoma / secretion* Islets of Langerhans / secretion Keto Acids / pharmacology* Kidney / physiology* Male Neoplasm Transplantation Pancreatic Neoplasms / secretion* Radioimmunoassay Rats Somatostatin / secretion |
| Grant Support | |
ID/Acronym/Agency:
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AM 07314/AM/NIADDK NIH HHS; AM 19525/AM/NIADDK NIH HHS; AM 22122/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Keto Acids; 11061-68-0/Insulin; 14930-96-2/Cytochalasin B; 364-98-7/Diazoxide; 367-47-5/Glyceraldehyde; 50-99-7/Glucose; 51110-01-1/Somatostatin; 816-66-0/alpha-ketoisocaproic acid; 9007-92-5/Glucagon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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