Document Detail


Fucoganglioside alpha-fucosyl(alpha-galactosyl)-GM1: a novel member of lipid membrane microdomain components involved in PC12 cell neuritogenesis.
MedLine Citation:
PMID:  17608628     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to search for novel components of lipid membrane microdomains involved in neural signalling pathways, mAbs (monoclonal antibodies) were raised against the detergent-insoluble membrane fraction of PC12 (pheochromocytoma) cells. Among the 22 hybrid clones, mAb PR#1 specifically detected a fucoganglioside Fuc(Gal)-GM1 [a-fucosyl(a-galactosyl)-GM1], a ganglioside homologous with GM1a (II3NeuAc,GgOse4Cer), as a novel member of microdomain components with biological functions. In the presence of mAb PR#1 in the culture medium, the outgrowth of neurites was induced in PC12 cells in a dose-dependent manner, with no effects on cell proliferation, suggesting that Fuc(Gal)-GM1 is preferentially involved in PC12 cell neuritogenesis. Effects through Fuc(Gal)-GM1 were different from those through GM1a during differentiation, e.g. under PR#1 treatment on Fuc(Gal)-GM1, round cell bodies with thinner cell processes were induced, whereas treatment with CTB (cholera toxin B subunit), a specific probe for GM1a, produced flattened cell bodies with thicker pro-cesses. Molecular analysis demonstrated that the PR#1-Fuc(Gal)-GM1 pathway was associated with Fyn and Yes of the Src family of kinases, although Src itself was not involved. No association was found with TrkA (tropomyosin receptor kinase A) and ERKs (extracellular-signal-regulated kinases), which are responsible for GM1a-induced differentiation. From these findings, it is suggested that a fucoganglioside Fuc(Gal)-GM1 provides a functional platform distinct from that of GM1a for signal transduction in PC12 cell differentiation.
Authors:
Yasuhiro Yamazaki; Yasuhiro Horibata; Yasuko Nagatsuka; Yoshio Hirabayashi; Tsutomu Hashikawa
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  407     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-07     Completed Date:  2007-11-06     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  31-40     Citation Subset:  IM    
Affiliation:
Laboratory for Neural Architecture, RIKEN Brain Science Institute, Wako, Saitama 351-0198, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / metabolism
G(M1) Ganglioside / analogs & derivatives*,  analysis,  metabolism
Gangliosides / metabolism*
Immunohistochemistry
Membrane Lipids / chemistry,  metabolism*
Mice
Mice, Inbred BALB C
Neurites / metabolism*,  ultrastructure
PC12 Cells
Rats
Signal Transduction
Spectrometry, Mass, Fast Atom Bombardment
Time Factors
src-Family Kinases / metabolism
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Gangliosides; 0/Membrane Lipids; 0/fucogangliosides; 0/fucosyl(galactosyl)-ganglioside GM1; 0/ganglioside GM1alpha; 37758-47-7/G(M1) Ganglioside; EC 2.7.10.2/src-Family Kinases
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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