Document Detail


Fuchsin fluorescence and autofluorescence in Cryptosporidium, Isospora and Cyclospora oocysts.
MedLine Citation:
PMID:  9925267     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cryptosporidium parvum and Isospora belli oocysts stained with carbol-fuchsin, as in a modified Ziehl Neelsen technique, fluoresce bright red under green light (546nm). Cryptosporidium oocysts tend to fluoresce more brightly the less intensely stained they appear under transmitted light; this is not the case with Isospora. Fuchsin-stained Cyclospora cayetanensis oocysts fluoresce rather dimly, but those not taking the dye retain their typical autofluorescence. Cryptosporidium and Isospora oocysts are also autofluorescent, appearing violet under u.v. light (365 nm), and green under violet (405 nm) and blue-violet light (436 nm). Their autofluorescence does not survive the staining procedure.
Authors:
M Varea; A Clavel; O Doiz; F J Castillo; M C Rubio; R Gómez-Lus
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal for parasitology     Volume:  28     ISSN:  0020-7519     ISO Abbreviation:  Int. J. Parasitol.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-03-09     Completed Date:  1999-03-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0314024     Medline TA:  Int J Parasitol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1881-3     Citation Subset:  IM    
Affiliation:
Servicio de Microbiología, Hospital Clínico Universitario, Zaragoza, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Coccidia / growth & development,  isolation & purification*
Coccidiosis / parasitology
Cryptosporidiosis / parasitology
Cryptosporidium parvum / growth & development,  isolation & purification*
Feces / parasitology*
Fluorescence
Humans
Isospora / growth & development,  isolation & purification*
Microscopy, Fluorescence
Rosaniline Dyes*
Species Specificity
Staining and Labeling / methods*
Chemical
Reg. No./Substance:
0/Rosaniline Dyes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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