Document Detail


FtsK functions in the processing of a Holliday junction intermediate during bacterial chromosome segregation.
MedLine Citation:
PMID:  11114887     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In bacteria with circular chromosomes, homologous recombination can generate chromosome dimers that cannot be segregated to daughter cells at cell division. Xer site-specific recombination at dif, a 28-bp site located in the replication terminus region of the chromosome, converts dimers to monomers through the sequential action of the XerC and XerD recombinases. Chromosome dimer resolution requires that dif is positioned correctly in the chromosome, and the activity of FtsK, a septum-located protein that coordinates cell division with chromosome segregation. Here, we show that cycles of XerC-mediated strand exchanges form and resolve Holliday junction intermediates back to substrate irrespective of whether conditions support a complete recombination reaction. The C-terminal domain of FtsK is sufficient to activate the exchange of the second pair of strands by XerD, allowing both intra- and intermolecular recombination reactions to go to completion. Proper positioning of dif in the chromosome and of FtsK at the septum is required to sense the multimeric state of newly replicated chromosomes and restrict complete Xer reactions to dimeric chromosomes.
Authors:
F X Barre; M Aroyo; S D Colloms; A Helfrich; F Cornet; D J Sherratt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genes & development     Volume:  14     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2000-12-26     Completed Date:  2001-01-18     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2976-88     Citation Subset:  IM    
Affiliation:
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, OX1 3QU, UK.
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MeSH Terms
Descriptor/Qualifier:
Chromosome Segregation / physiology*
Chromosomes, Bacterial / physiology*
Cytoplasm / metabolism
DNA Nucleotidyltransferases / metabolism*
Escherichia coli Proteins
Integrases*
Membrane Proteins / genetics,  metabolism*
Recombinases
Recombination, Genetic
Chemical
Reg. No./Substance:
0/Escherichia coli Proteins; 0/FtsK protein, E coli; 0/Membrane Proteins; 0/Recombinases; EC 2.7.7.-/DNA Nucleotidyltransferases; EC 2.7.7.-/Integrases; EC 2.7.7.-/integron integrase IntI1
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