| Frozen competent yeast cells that can be transformed with high efficiency using the LiAc/SS carrier DNA/PEG method. | |
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MedLine Citation:
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PMID: 17401330 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Here we describe a protocol for the production of frozen competent yeast cells that can be transformed with high efficiency using the lithium acetate/single-stranded carrier DNA/PEG method. This protocol allows the production of highly competent yeast cells that can be frozen and used at a later date and is especially useful for laboratories using one or two strains repeatedly. The production of yeast cells for freezing takes only approximately 30 min, once the yeast culture has grown up. Transformation with frozen competent yeast cells will take approximately 30 min, depending on the heat shock used. |
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Authors:
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R Daniel Gietz; Robert H Schiestl |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nature protocols Volume: 2 ISSN: 1750-2799 ISO Abbreviation: Nat Protoc Publication Date: 2007 |
Date Detail:
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Created Date: 2007-04-02 Completed Date: 2007-06-29 Revised Date: 2008-03-24 |
Medline Journal Info:
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Nlm Unique ID: 101284307 Medline TA: Nat Protoc Country: England |
Other Details:
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Languages: eng Pagination: 1-4 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetates Cell Culture Techniques / methods* Cryopreservation / methods* DNA, Single-Stranded / genetics Polyethylene Glycols Saccharomyces cerevisiae / cytology, genetics* Transformation, Genetic* |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/DNA, Single-Stranded; 0/Polyethylene Glycols; 0/lithium acetate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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