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Frozen competent yeast cells that can be transformed with high efficiency using the LiAc/SS carrier DNA/PEG method.
MedLine Citation:
PMID:  17401330     Owner:  NLM     Status:  MEDLINE    
Here we describe a protocol for the production of frozen competent yeast cells that can be transformed with high efficiency using the lithium acetate/single-stranded carrier DNA/PEG method. This protocol allows the production of highly competent yeast cells that can be frozen and used at a later date and is especially useful for laboratories using one or two strains repeatedly. The production of yeast cells for freezing takes only approximately 30 min, once the yeast culture has grown up. Transformation with frozen competent yeast cells will take approximately 30 min, depending on the heat shock used.
R Daniel Gietz; Robert H Schiestl
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nature protocols     Volume:  2     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2007  
Date Detail:
Created Date:  2007-04-02     Completed Date:  2007-06-29     Revised Date:  2008-03-24    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  1-4     Citation Subset:  IM    
Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.
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MeSH Terms
Cell Culture Techniques / methods*
Cryopreservation / methods*
DNA, Single-Stranded / genetics
Polyethylene Glycols
Saccharomyces cerevisiae / cytology,  genetics*
Transformation, Genetic*
Reg. No./Substance:
0/Acetates; 0/DNA, Single-Stranded; 0/Polyethylene Glycols; 0/lithium acetate

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