| Frontiers of mass spectrometry in nucleic acids analysis. | |
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MedLine Citation:
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PMID: 20530841 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleic acids research is a highly competitive field of research. A number of well established methods are available. The current output of high throughput ("next generation") sequencing technologies is impressive, and still technologies are continuing to make progress regarding read lengths, bp per second, accuracy and costs. Although in the 1990s MS was considered as an analytical platform for sequencing, it was soon realized that MS will never be competitive. Thus, the focus shifted from de novo sequencing towards other areas of application where MS has proven to be a powerful analytical tool. Potential niches for the application of MS in nucleic acids research include genotyping of genetic markers (single nucleotide polymorphisms, short tandem repeats, and combinations thereof), quality control of synthetic oligonucleotides, metabolic profiling of therapeutics, characterization of modified nucleobases in DNA and RNA molecules, and the study of non covalent interactions among nucleic acids as well as interactions of nucleic acids with drugs and proteins. The diversity of possible applications for MS highlights its significance for nucleic acid research. |
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Authors:
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Herbert Oberacher |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: European journal of mass spectrometry (Chichester, England) Volume: 16 ISSN: 1469-0667 ISO Abbreviation: Eur J Mass Spectrom (Chichester, Eng) Publication Date: 2010 |
Date Detail:
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Created Date: 2010-06-09 Completed Date: 2010-08-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101124748 Medline TA: Eur J Mass Spectrom (Chichester, Eng) Country: England |
Other Details:
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Languages: eng Pagination: 351-65 Citation Subset: IM |
Affiliation:
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Institute of Legal Medicine, Innsbruck Medical University, Innsbruck, Austria. herbert.oberacher@i-med.ac.at |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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DNA Methylation Genetic Markers Genome Mass Spectrometry / methods*, trends* Nucleic Acids / analysis*, chemistry, metabolism Oligonucleotides / analysis, chemistry RNA / genetics, metabolism RNA Processing, Post-Transcriptional |
| Chemical | |
Reg. No./Substance:
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0/Genetic Markers; 0/Nucleic Acids; 0/Oligonucleotides; 63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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