Document Detail


From proton nuclear magnetic resonance spectra to pH. Assessment of 1H NMR pH indicator compound set for deuterium oxide solutions.
MedLine Citation:
PMID:  19616695     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, a protocol for pH determination from D(2)O samples using (1)H NMR pH indicator compounds was developed and assessed by exploring the pH-dependency of 13 compounds giving pH-dependent (1)H NMR signals. The indicators cover the pH range from pH(*) 0 to 7.2. Equations to transform the indicator chemical shifts to pH estimates are given here for acetic acid, formic acid, chloroacetic acid, dichloroacetic acid, creatine, creatinine, glycine, histidine, 1,2,4-triazole, and TSP (2,2,3,3-tetradeutero-3-(trimethylsilyl)-propionic acid). To characterize the method in presence of typical solutes, the effects of common metabolites, albumin and ionic strength were also evaluated. For the ionic strengths, the effects were also modelled. The experiments showed that the use of pH sensitive (1)H NMR chemical shifts allows the pH determination of typical metabolite solutions with accuracy of 0.01-0.05 pH units. Also, when the ionic strength is known with accuracy better than 0.1 mol dm(-3) and the solute concentrations are low, pH(nmr)(*) (the NMR estimate of pH) can be assumed to be within 0.05 pH units from potentiometrically determined pH.
Authors:
Tuulia Tynkkynen; Mika Tiainen; Pasi Soininen; Reino Laatikainen
Related Documents :
16416175 - Regional postprandial differences in ph within the stomach and gastroesophageal junction.
6273445 - The in-vitro degradation of poly(glycolic acid) sutures--effect of ph.
8885415 - Acid tolerance in listeria monocytogenes: the adaptive acid tolerance response (atr) an...
24092895 - Anti-glutamic acid decarboxylase antibody associated limbic encephalitis in a child: ex...
11499915 - Bioconversion of limonene to increased concentrations of perillic acid by pseudomonas p...
3936815 - In vivo anti-allergic and anti-inflammatory effects of drugs that inhibit lipoxygenase ...
Publication Detail:
Type:  Journal Article     Date:  2009-06-24
Journal Detail:
Title:  Analytica chimica acta     Volume:  648     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-20     Completed Date:  2009-09-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  105-12     Citation Subset:  IM    
Affiliation:
Laboratory of Chemistry, Department of Biosciences, University of Kuopio, 70211 Kuopio, Finland. tuulia.tynkkynen@uku.fi
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Deuterium Oxide / chemistry*
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy / methods*
Organic Chemicals / analysis,  chemistry
Osmolar Concentration
Protons
Solutions
Chemical
Reg. No./Substance:
0/Organic Chemicals; 0/Protons; 0/Solutions; 7789-20-0/Deuterium Oxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Metabolomic profiling of human urine in hepatocellular carcinoma patients using gas chromatography/m...
Next Document:  Determination of marker pteridins and biopterin reduced forms, tetrahydrobiopterin and dihydrobiopte...