Document Detail


From c-Photina mouse embryonic stem cells to high-throughput screening of differentiated neural cells via an intermediate step enriched in neural precursor cells.
MedLine Citation:
PMID:  20834010     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of engineered mouse embryonic stem (mES) cells in high-throughput screening (HTS) can offer new opportunities for studying complex targets in their native environment, increasing the probability of discovering more meaningful hits. The authors have generated and developed a mouse embryonic stem cell line called c-Photina mES stably expressing a Ca(2+)-activated photoprotein as a reporter gene. This reporter cell line retains the ability to differentiate into any cell lineage and can be used for miniaturized screening processes in 384-well microplates. The c-Photina mES cell line is particularly well suited for the study of the pharmacological modulation of target genes that induce Ca(2+) mobilization. The authors differentiated this mES reporter cell line into neuronal cells and screened the LOPAC(1280) library monitoring the agonistic or antagonistic activities of compounds. They also demonstrate the possibility to generate and freeze bulk preparations of cells at an intermediate stage of differentiation and enriched in neural precursor cells, which retain the ability to form fully functional neural networks once thawed. The proposed cell model is of high value for HTS purposes because it offers a more physiological environment to the targets of interest and the possibility of using frozen batches of neural precursor cells.
Authors:
Silvia Cainarca; Simone Fenu; Silvia Bovolenta; Patrizia Arioli; Andrea Menegon; Stefan Lohmer; Sabrina Corazza
Publication Detail:
Type:  Journal Article     Date:  2010-09-10
Journal Detail:
Title:  Journal of biomolecular screening     Volume:  15     ISSN:  1552-454X     ISO Abbreviation:  J Biomol Screen     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-08     Completed Date:  2011-01-20     Revised Date:  2011-05-23    
Medline Journal Info:
Nlm Unique ID:  9612112     Medline TA:  J Biomol Screen     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1132-43     Citation Subset:  IM    
Affiliation:
AXXAM SpA, Milan, Italy. silvia.cainarca.sc@axxam.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Assay
Cell Culture Techniques / methods*
Cell Differentiation* / drug effects
Cell Line
Embryonic Stem Cells / cytology*,  drug effects,  metabolism
High-Throughput Screening Assays / methods*
Mice
Neural Stem Cells / cytology*,  drug effects,  metabolism
Neurons / cytology*,  drug effects,  metabolism
Pyridinium Compounds / metabolism
Quaternary Ammonium Compounds / metabolism
Small Molecule Libraries / analysis,  pharmacology
Chemical
Reg. No./Substance:
0/FM 4-64; 0/Pyridinium Compounds; 0/Quaternary Ammonium Compounds; 0/Small Molecule Libraries

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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