Document Detail

From HeLa cell division to infectious diarrhoea.
MedLine Citation:
PMID:  1964248     Owner:  NLM     Status:  MEDLINE    
Hela S3 cells were grown in suspension both randomly and, synchronously using hydroxyurea which blocks cells at the G1/S interface. Cryosections were prepared, freeze-dried and analyzed by X-ray microanalysis. As cells moved into S and through M phases [Na] and [Cl] increased; both returned to normal levels upon re-entering G1 phase. The Na/K ratio was 1:1 in G1 phase. Infection of HeLa S3 cells in G1 phase with vaccinia virus resulted in no change in intracellular [Na]. Infection of neonatal mice with murine rotavirus was localized to villus tip enterocytes and gave rise to diarrhoea which was maximal at 72h post-infection (p.i.). Diarrhoea was preceded by ischemia of villi (18-42h p.i.) and villus shortening (maximal at 42h p.i.), and was also coincident with a dramatic regrowth of villi. At 48h p.i. a proliferative zone of electron lucent cells was observed in villus base regions. Cryosections of infected gut, taken before, during, and after infection, together with corresponding age-matched controls, were freeze-dried and analysed by X-ray microanalysis. At 48h p.i. electron lucent villus base cells were shown to be more hydrated, and, to contain higher levels of both Na and Cl and lower levels of P, S, K and Mg than corresponding control cells. These studies (we argue) increase confidence in the use of X-ray microanalysis in studying biological systems, provide some insight into the process of cell division, and constitute the basis of a new concept of diarrhoeal secretion.
J Stephen; M P Osborne; A J Spencer; A Warley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Scanning microscopy     Volume:  4     ISSN:  0891-7035     ISO Abbreviation:  Scanning Microsc.     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1991-05-02     Completed Date:  1991-05-02     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  8704616     Medline TA:  Scanning Microsc     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  781-6     Citation Subset:  IM    
Department of Microbiology, University of Birmingham, U.K.
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MeSH Terms
Cell Division
Chlorides / analysis
Diarrhea / microbiology,  pathology*
Digestive System / chemistry,  microbiology,  pathology
Electron Probe Microanalysis / methods*
Hela Cells / cytology*,  physiology
Rotavirus / isolation & purification
Sodium / analysis
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Chlorides; 7440-23-5/Sodium

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