| From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. | |
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MedLine Citation:
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PMID: 22245580 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Signaling photoreceptors mediate diverse organismal adaptations in response to light. As light-gated protein switches, signaling photoreceptors provide the basis for optogenetics, a term that refers to the control of organismal physiology and behavior by light. We establish as novel optogenetic tools the plasmids pDusk and pDawn, which employ blue-light photoreceptors to confer light-repressed or light-induced gene expression in Escherichia coli with up to 460-fold induction upon illumination. Key features of these systems are low background activity, high dynamic range, spatial control on the 20-μm scale, independence from exogenous factors, and ease of use. In optogenetic experiments, pDusk and pDawn can be used to specifically perturb individual nodes of signaling networks and interrogate their role. On the preparative scale, pDawn can induce by light the production of recombinant proteins and thus represents a cost-effective and readily automated alternative to conventional induction systems. |
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Authors:
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Robert Ohlendorf; Roee R Vidavski; Avigdor Eldar; Keith Moffat; Andreas Möglich |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-8 |
Journal Detail:
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Title: Journal of molecular biology Volume: - ISSN: 1089-8638 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985088R Medline TA: J Mol Biol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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Humboldt-Universität zu Berlin, Institut für Biologie, Biophysikalische Chemie, Invalidenstraße 42, 10115 Berlin, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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