Document Detail

Frigen II improves the reliability of measurement of interleukin-1 related substances in amniotic fluid.
MedLine Citation:
PMID:  10203300     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: To investigate the role of interleukin-1 related substances in amniotic fluid in normal term labor without intrauterine infection. METHODS: Amniotic fluid samples were collected from forty-one patients with various backgrounds. A novel pre-assay treatment using Frigen II was introduced to improve the recovery rates of cytokines, i.e., interleukin-1alpha, interleukin-1beta and interleukin-1 receptor antagonist, prior to ELISA assay. Urine samples from newborn infants were also tested. RESULTS: The concentrations of interleukin-1alpha, interleukin-1beta and interleukin-1ra were significantly higher in samples from normal vaginal delivery. The higher levels of interleukin-1alpha were also observed in samples from preterm labor without infection. Preterm infants produced more of interleukin-1 receptor antagonist in urine compared with term infants. CONCLUSIONS: This study provides evidence of the possible involvement of interleukin-1 related substances in labor without signs of infection. The data from newborn urine suggests that amniotic interleukin-1ra originates from the fetus. However, amniotic fluid interleukin-1alpha and interleukin-1beta may be derived from maternal tissue, such as decidua.
O Ishihara; M Saitoh; K Kinoshita; H Takanashi; Y Arai; H Kitagawa
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta obstetricia et gynecologica Scandinavica     Volume:  78     ISSN:  0001-6349     ISO Abbreviation:  Acta Obstet Gynecol Scand     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-04-29     Completed Date:  1999-04-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370343     Medline TA:  Acta Obstet Gynecol Scand     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  321-5     Citation Subset:  IM    
Department of Obstetrics & Gynecology, Saitama Medical Center, Saitama Medical School, Kawagoe, Japan.
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MeSH Terms
Amniotic Fluid / chemistry*
Chlorofluorocarbons, Methane
Enzyme-Linked Immunosorbent Assay
Infant, Newborn
Interleukin 1 Receptor Antagonist Protein
Interleukin-1 / analysis*
Labor, Obstetric / metabolism
Obstetric Labor, Premature / metabolism
Reagent Kits, Diagnostic
Receptors, Interleukin-1 / antagonists & inhibitors
Sialoglycoproteins / analysis
Reg. No./Substance:
0/Chlorofluorocarbons, Methane; 0/IL1RN protein, human; 0/Interleukin 1 Receptor Antagonist Protein; 0/Interleukin-1; 0/Reagent Kits, Diagnostic; 0/Receptors, Interleukin-1; 0/Sialoglycoproteins; 75-45-6/fluorocarbon 22

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