| Increased friction coefficient and superficial zone protein expression in patients with advanced osteoarthritis. | |
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MedLine Citation:
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PMID: 20499384 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To quantify the concentration of superficial zone protein (SZP) in the articular cartilage and synovial fluid of patients with advanced osteoarthritis (OA) and to further correlate the SZP content with the friction coefficient, OA severity, and levels of proinflammatory cytokines. METHODS: Samples of articular cartilage and synovial fluid were obtained from patients undergoing elective total knee replacement surgery. Additional normal samples were obtained from donated body program and tissue bank sources. Regional SZP expression in cartilage obtained from the femoral condyles was quantified by enzyme-linked immunosorbent assay (ELISA) and visualized by immunohistochemistry. Friction coefficient measurements of cartilage plugs slid in the boundary lubrication system were obtained. OA severity was graded using histochemical analyses. The concentrations of SZP and proinflammatory cytokines in synovial fluid were determined by ELISA. RESULTS: A pattern of SZP localization in knee cartilage was identified, with load-bearing regions exhibiting high SZP expression. SZP expression patterns were correlated with friction coefficient and OA severity; however, SZP expression was observed in all samples at the articular surface, regardless of OA severity. SZP expression and aspirate volume of synovial fluid were higher in OA patients than in normal controls. Expression of cytokines was elevated in the synovial fluid of some patients. CONCLUSION: Our findings indicate a mechanochemical coupling in which physical forces regulate OA severity and joint lubrication. The findings of this study also suggest that SZP may be ineffective in reducing joint friction in the boundary lubrication mode at an advanced stage of OA, where other mechanisms may dominate the observed tribological behavior. |
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Authors:
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C P Neu; A H Reddi; K Komvopoulos; T M Schmid; P E Di Cesare |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Arthritis and rheumatism Volume: 62 ISSN: 1529-0131 ISO Abbreviation: Arthritis Rheum. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-27 Completed Date: 2010-11-09 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 0370605 Medline TA: Arthritis Rheum Country: United States |
Other Details:
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Languages: eng Pagination: 2680-7 Citation Subset: AIM; IM |
Affiliation:
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Purdue University, West Lafayette, Indiana 47097-2032, USA. cpneu@purdue.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Cartilage, Articular / metabolism*, pathology, physiopathology Enzyme-Linked Immunosorbent Assay Female Fluorescent Antibody Technique, Indirect Friction / physiology Humans Immunoenzyme Techniques Knee Joint / metabolism*, pathology, physiopathology Lubrication Male Mechanotransduction, Cellular / physiology* Middle Aged Osteoarthritis, Knee / metabolism*, pathology, physiopathology Proteoglycans / metabolism* Stress, Mechanical Weight-Bearing / physiology |
| Grant Support | |
ID/Acronym/Agency:
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R01 AR050457-05/AR/NIAMS NIH HHS; R01-AR-050457/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/PRG4 protein, human; 0/Proteoglycans |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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