Document Detail


Frex and FrexH: Indicators of metabolic states in living cells.
MedLine Citation:
PMID:  22572785     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reduced nicotinamide adenine dinucleotide (NADH) and its oxidized form play central roles in energy and redox metabolisms. For many years, researchers have relied on the weak NADH endogenous fluorescence signal to determine the NADH level in living cells. We recently reported a series of genetically encoded fluorescent sensors highly specific for NADH. These sensors allow real-time, quantitative measurement of this significant molecule in different subcellular compartments. In this study, we provide a more detailed discussion of the benefits and limitations of these genetically encoded fluorescent sensors. These sensors are utilized in most laboratories without the need for sophisticated instruments because of their superior sensitivity and specificity. They are also viable alternatives to existing techniques for measuring the endogenous fluorescence of intracellular NAD(P)H.
Authors:
Yuzheng Zhao; Yi Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-01
Journal Detail:
Title:  Bioengineered bugs     Volume:  3     ISSN:  1949-1026     ISO Abbreviation:  Bioeng Bugs     Publication Date:    2012 May-Jun
Date Detail:
Created Date:  2012-06-08     Completed Date:  2012-10-03     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  101525147     Medline TA:  Bioeng Bugs     Country:  United States    
Other Details:
Languages:  eng     Pagination:  181-8     Citation Subset:  IM    
Affiliation:
Synthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
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MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques / methods*
Electron Transport / physiology
Fluorescence*
NAD / metabolism*
Chemical
Reg. No./Substance:
53-84-9/NAD
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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