| Frequent loss of heterozygosity in early non-small cell lung cancers at chromosome 9p21 proximal to the CDKN2a gene. | |
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MedLine Citation:
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PMID: 9139845 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Deletions involving the chromosome 9p21 region have been reported as frequent events in non-small cell lung cancer (NSCLC). To investigate potential tumor-suppressor gene (TSG) loci within the 9p21 region, which also harbors the candidate TSG locus CDKN2a, we studied 32 cases of primary NSCLC for loss of heterozygosity (LOH). Tumor and paired normal lung cells were microdissected from lung tissue imprints and all samples screened using PCR-LOH analysis with 15 9p markers. In addition, 3 NSCLC cell lines and their matched normal lung and tumor DNA were similarly analyzed. LOH at the marker D9S259, which is proximal to the CDKN2a locus, was found most frequently (52%), while LOH at D9S942, the marker closest (5 kb) to the CDKN2a gene, was seen in only 17%. Homozygous loss of markers close to CDKN2a was, however, detected in 2 of the 3 cell lines and one accompanying tumor sample. We propose that a TSG in the region of deletion proximal to the CDKN2a gene within 9p21 may play a significant role in the pathogenesis and progression of NSCLC. |
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Authors:
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L J Mead; M T Gillespie; J Y Hung; U S Rane; K C Rayeroux; L B Irving; L J Campbell |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: 71 ISSN: 0020-7136 ISO Abbreviation: Int. J. Cancer Publication Date: 1997 Apr |
Date Detail:
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Created Date: 1997-05-23 Completed Date: 1997-05-23 Revised Date: 2007-07-24 |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 213-7 Citation Subset: IM |
Affiliation:
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Victorian Cancer Cytogenetics Service, St. Vincent's Hospital, Fitzroy, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Non-Small-Cell Lung
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genetics* Carrier Proteins / genetics* Chromosome Deletion* Chromosomes, Human, Pair 9 / genetics* Cyclin-Dependent Kinase Inhibitor p16 DNA, Neoplasm / analysis Genes, Tumor Suppressor / genetics Heterozygote* Humans Lung / physiology Lung Neoplasms / genetics* Microsatellite Repeats Polymerase Chain Reaction Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Cyclin-Dependent Kinase Inhibitor p16; 0/DNA, Neoplasm |
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