| Frequent detection of tumor cells in hematopoietic grafts in neuroblastoma and Ewing's sarcoma. | |
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MedLine Citation:
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PMID: 9849694 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Many poor-risk neuroblastomas and tumours of the Ewing's sarcoma family (ET) recur despite autologous transplants. Recurrence may be due to tumor cells contained in the BM harvests or PBSC harvests. The objectives of this prospective study were to: (1) determine the incidence and degree of tumor cell contamination in paired BM and PBSC harvests; and (2) determine the efficacy of tumor cell purging by immunomagnetic CD34+ cell selection. 198 samples from 11 consecutive patients with neuroblastoma or Ewing's sarcoma were analyzed. We assayed tumor contamination by RT-PCR assay for PGP 9.5, plus immunohistochemistry for neuroblastoma-specific antigens (the latter in neuroblastoma only). None of these patients had tumor cells detected in their BM by clinical histology immediately before BM or PBSC harvests. However, 82% of PBSC and 89% of backup BM harvests were contaminated with tumor by RT-PCR and/or immunocytochemistry assays. Unselected PBSC and BM harvests contained similar quantities of tumor cells (median, approximately 200000 cells). Cyclophosphamide plus G-CSF mobilization did not affect the incidence or level of contamination in PBSC harvests, as compared to blood obtained before mobilization. Immunomagnetic CD34+ cell selection depleted tumor cells by a median of 3.0 logs for PBSC, and 2.6 logs for BM harvests. |
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Authors:
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W Leung; A R Chen; R C Klann; T J Moss; J M Davis; S J Noga; K J Cohen; A D Friedman; D Small; C L Schwartz; M J Borowitz; M D Wharam; C N Paidas; C A Long; S Karandish; J D McMannis; M B Kastan; C I Civin |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bone marrow transplantation Volume: 22 ISSN: 0268-3369 ISO Abbreviation: Bone Marrow Transplant. Publication Date: 1998 Nov |
Date Detail:
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Created Date: 1999-02-16 Completed Date: 1999-02-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8702459 Medline TA: Bone Marrow Transplant Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 971-9 Citation Subset: IM |
Affiliation:
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Johns Hopkins Oncology Center, Baltimore MD, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Antigens, CD34 Child Child, Preschool Cyclophosphamide / therapeutic use Disease Progression Female Granulocyte Colony-Stimulating Factor / therapeutic use Hematopoietic Stem Cell Mobilization Hematopoietic Stem Cell Transplantation* Hematopoietic Stem Cells / pathology* Humans Immunomagnetic Separation* Immunosuppressive Agents / therapeutic use Male Neuroblastoma / pathology*, therapy* Polymerase Chain Reaction Prospective Studies Recurrence Sarcoma, Ewing's / pathology*, therapy* Survival Rate Transplantation, Autologous |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD34; 0/Immunosuppressive Agents; 143011-72-7/Granulocyte Colony-Stimulating Factor; 50-18-0/Cyclophosphamide |
| Comments/Corrections | |
Comment In:
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Bone Marrow Transplant. 1999 Jul;24(2):229-30
[PMID:
10455360
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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