Document Detail

Frequent deletions of material from chromosome arm 1p in oligodendroglial tumors revealed by double-target fluorescence in situ hybridization and microsatellite analysis.
MedLine Citation:
PMID:  8605118     Owner:  NLM     Status:  MEDLINE    
We undertook a cytogenetic analysis of 29 human brain tumors using double-target fluorescence in situ hybridization (FISH) and focusing on chromosome arm 1p. One or more tumor suppressor genes in this arm have been suggested to be important in a variety of neuroectodermal tumors. The series included 9 oligodendrogliomas, 4 mixed gliomas, 10 astrocytomas, 4 glioblastomas, and 2 central neurocytomas. We hybridized pericentromeric (1q12) and subtelomeric (1p36) DNA probes to cell nuclei prepared from paraffin-embedded tissues and observed a strikingly high incidence of deletion of at least part of 1p in oligodendrogliomas (100%) and mixed gliomas (75%). The results of the FISH analyses were confirmed by demonstration of loss of heterozygosity for a microsatellite polymorphism in 10 of the 29 tumors. As well as supporting the feasibility of FISH for detecting allelic deletions in chromosomes from paraffin-embedded tumor samples, the alteration of 1p reported here will contribute to an understanding of the molecular genetic events in oligodendroglial tumor development.
N Hashimoto; D Ichikawa; Y Arakawa; K Date; S Ueda; Y Nakagawa; A Horii; Y Nakamura; T Abe; J Inazawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genes, chromosomes & cancer     Volume:  14     ISSN:  1045-2257     ISO Abbreviation:  Genes Chromosomes Cancer     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-05-21     Completed Date:  1996-05-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9007329     Medline TA:  Genes Chromosomes Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  295-300     Citation Subset:  IM    
Department of Hygiene, Kyoto Prefectural University of Medicine, Japan.
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MeSH Terms
Brain Neoplasms / genetics*
Chromosome Aberrations*
Chromosomes, Human, Pair 1*
Glioma / genetics*
In Situ Hybridization, Fluorescence
Microsatellite Repeats
Oligodendroglia / pathology*
Sequence Deletion

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