| Frequent cholesterol intake up-regulates intestinal NPC1L1, ACAT2, and MTP. | |
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MedLine Citation:
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PMID: 20405839 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dietary cholesterol elevates plasma total cholesterol (TC) level. However, no study to date has examined how cholesterol intake frequency interacts with the gene of sterol transporters, receptors, and enzymes involved in cholesterol metabolism. Thirty-three hamsters were divided into three groups with the control hamsters being given daily 9 mg of cholesterol in the diet (CD), whereas the second group being gavage-administered 3 mg of cholesterol three times per day (C-3) and the third group being gavage-administered 9 mg of cholesterol one time per day (C-1). The experiment lasted for 6 weeks. The hamsters were killed under carbon dioxide suffocation. Data demonstrated that plasma TC, non-high-density lipoprotein cholesterol, and triacylglycerols were elevated with the increasing cholesterol intake frequency. Western blotting analyses revealed that the intake frequency had no effect on protein mass of hepatic sterol regulatory element binding protein-2, liver X receptor-alpha, 3-hydroxy-3-methylglutaryl-CoA reductase, LDL receptor, and cholesterol-7alpha-hydroxylase. However, the frequent cholesterol intake down-regulated the mRNA level of hepatic LDL receptor. In contrast, the frequent cholesterol intake up-regulated the mRNA levels of intestinal Niemann-Pick C1-like 1 (NPC1L1), acyl coenzyme A:cholesterol acyltransferase 2 (ACAT2), and microsomal triacylglycerol transport protein (MTP). It was concluded that the cholesterol intake frequency-induced elevation in plasma TC was associated with greater cholesterol absorption, possibly mediated by up-regulation of NPC1L1, ACAT2, and MTP. |
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Authors:
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Rui Jiao; Lei Guan; Nan Yang; Cheng Peng; Yintong Liang; Ka Ying Ma; Yu Huang; Zhen-Yu Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 58 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-05 Completed Date: 2010-08-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 5851-7 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Chinese University of Hong Kong, Shatin, NT, Hong Kong, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Cholesterol / metabolism* Cricetinae Intestines / metabolism* Male Membrane Proteins / genetics, metabolism* Mesocricetus Up-Regulation* |
| Chemical | |
Reg. No./Substance:
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0/Membrane Proteins; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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