Document Detail


Frequency of thrombophilia-related genetic variations in patients with idiopathic pulmonary embolism in an urban emergency department.
MedLine Citation:
PMID:  16574759     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The frequency of the thrombophilic genetic variants factor V Leiden (FVL) G1691A, prothrombin G20210A, and methylenetetrahydrofolate reductase (MTHFR) C677T in acutely symptomatic ambulatory patients with idiopathic pulmonary embolism (PE) has not been measured. METHODS: This prospective case-control study included patients presenting to urban emergency departments (EDs) with chest pain or shortness of breath. Cases were classified as idiopathic PE (49 patients with PE, but without overt risk factors for thrombosis). Control groups included (a) patients with nonidiopathic PE (152 patients with PE and risk factors); (b) patients in whom PE was excluded (91 patients who had PE ruled out with a structured protocol, including follow-up); and (c) patients in whom PE was not suspected (193 patients without a workup for PE, who were free of PE on follow-up). Blood DNA extracts were analyzed by PCR and restriction fragment length polymorphism analysis for the FVL, prothrombin, and MTHFR sequence variations. RESULTS: Either the FVL or prothrombin variant was found in 10% (95% confidence interval, 3%-22%) of patients with idiopathic PE compared with 13% (8%-20%) of nonidiopathic PE, 2% (5%-14%) of PE excluded, and 9% (5%-14%) of PE not suspected patients. Patients with idiopathic PE tended to have a higher frequency of homozygous MTHFR sequence variants, but mean (SD) plasma homocysteine concentrations were not increased [15.6 (5.4) micromol/L vs 12.8 (4.6) micromol/L for homozygous, and wild-type, respectively; P = 0.40]. CONCLUSIONS: The frequency of either the FVL or prothrombin sequence variant was not increased in idiopathic PE patients compared with nonidiopathic PE patients or patients who had PE excluded. These data suggest that genotyping to detect idiopathic PE would have limited clinical utility in the urban ED setting.
Authors:
Lori Kruse; Alice M Mitchell; Carlos A Camargo; Jackeline Hernandez; Jeffrey A Kline
Related Documents :
18705289 - Approach to patients with pulmonary embolism in a surgical intensive care unit.
79229 - Beta-thromboglobulin and deep vein thrombosis.
12540959 - Ruling out clinically suspected pulmonary embolism by assessment of clinical probabilit...
3614469 - Risk evaluation of thromboembolic diseases according to functional test data in patient...
9855319 - In unfractionated heparin dosing, the combination of patient age and estimated plasma v...
14608129 - Use of lepirudin during percutaneous vascular interventions in patients with heparin-in...
321709 - A double-blind comparison of clindamycin with penicillin plus chloramphenicol in treatm...
9247979 - Behavioral, sympathetic and adrenocortical responses to yohimbine in panic disorder pat...
9181659 - Isolation of dysgonic fermenter 3, a rare isolate associated with diarrhoea in immunoco...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-03-30
Journal Detail:
Title:  Clinical chemistry     Volume:  52     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-25     Completed Date:  2006-06-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1026-32     Citation Subset:  IM    
Affiliation:
Department of Emergency Medicine, James G. Cannon Research Center, Carolinas Medical Center, Charlotte, NC 28203, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acute Disease
Case-Control Studies
Coronary Disease / genetics
Diagnosis, Differential
Emergency Service, Hospital
Factor V / genetics
Female
Genetic Variation
Genotype
Humans
Hyperhomocysteinemia / genetics
Male
Methylenetetrahydrofolate Reductase (NADPH2) / genetics
Middle Aged
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Prospective Studies
Prothrombin / genetics
Pulmonary Embolism / genetics*
Syndrome
Thrombophilia / genetics*
Grant Support
ID/Acronym/Agency:
R01 HL074384/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/factor V Leiden; 9001-24-5/Factor V; 9001-26-7/Prothrombin; EC 1.5.1.20/Methylenetetrahydrofolate Reductase (NADPH2)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Anti-cardiolipin antibodies and overall survival in a large cohort: preliminary report.
Next Document:  Quantification of fragments of human serum inter-alpha-trypsin inhibitor heavy chain 4 by a surface-...