Document Detail


Frequency of satellite association of human chromosomes is correlated with amount of Ag-staining of the nucleolus organizer region.
MedLine Citation:
PMID:  70995     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Methaphase chromosomes from karyotypically normal adult humans (three males, six females) and one male with a 13p - chromosome were stained by quinacrine and then by the Ag-AS silver staining method to reveal nucleolus organizer regions (NORs). Each person had a characteristic number of Ag-stained chromosomes per cell, always fewer than 10. Determination of the mean Ag-size of each chromosome showed that each of the 10 individuals had a unique distribution of Ag-stain. Within each individual, there was some variation from cell to cell in the number of acrocentric chromosomes that were Ag-stained; this was not random, and the same chromosomes (those that had at most a small amount of Ag-stain) tended to be unstained in every cell. Satellite associations were scored on the same cells. Chromosomes that had no Ag-stain were involved in satellite association less than 20% as often as those that had some Ag-stain. Chromosomes that had a small amount of Ag-stain were involved in association about 50% as often as those that had a large amount of stain. Regression analysis of the 50 (of a total of 100) acrocentric chromosomes which could be individually identified by quinacrine markers showed that the frequency with which a chromosome was involved in satellite association was strongly correlated with the amount of Ag-stained material in the NOR.
Authors:
D A Miller; R Tantravahi; V G Dev; O J Miller
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of human genetics     Volume:  29     ISSN:  0002-9297     ISO Abbreviation:  Am. J. Hum. Genet.     Publication Date:  1977 Sep 
Date Detail:
Created Date:  1977-10-28     Completed Date:  1977-10-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370475     Medline TA:  Am J Hum Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  490-502     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleolus*
Chromosome Deletion
Chromosomes / ultrastructure*
Cytological Techniques
DNA*
DNA, Satellite*
Female
Humans
Karyotyping
Male
Quinacrine
Silver
Staining and Labeling
Chemical
Reg. No./Substance:
0/DNA, Satellite; 7440-22-4/Silver; 83-89-6/Quinacrine; 9007-49-2/DNA
Comments/Corrections

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