Document Detail


Frequency and quantity of the parvovirus B19 genome in endomyocardial biopsies from patients with suspected myocarditis or idiopathic left ventricular dysfunction.
MedLine Citation:
PMID:  15085375     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Parvovirus B19 (PB19) has been identified as a possible cause of myocarditis and heart failure in both children and adult patients. This study used real time PCR analysis, to determine the frequency and to quantify PB19 viral genomes in endomyocardial tissue samples from 80 adult patients with clinically suspected myocarditis or idiopathic left ventricular dysfunction and from 36 controls. Histological (Dallas classification) and immunohistological analyses were performed to detect myocardial inflammation in the endomyocardial biopsies.PB19 genomic DNA was found in nine of 80 patients (11.2%), 4 out of 31 (12.9%) patients with inflammatory infiltrates detected via immunohistological methods and 5 out of 49 (10.2%) patients with left ventricular dysfunction without myocardial inflammation. The copy numbers for PB19 DNA ranged between 30 and 3900 per microg of cellular DNA. Four patients with clinically suspected myocarditis had copy numbers for PB19 DNA of 70, 740, 3400 and 3900, respectively, per microg of cellular DNA in the endomyocardial biopsy. Five patients with idiopathic left ventricular dysfunction had copy numbers for PB19 DNA of 30, 38, 52, 58 and 90, respectively, per microg of cellular DNA in the endomyocardial biopsy. The amplicon of one of the nine positive PCR fragment was sequenced and was found to be fully identical in the highly conserved sequence of published Parvovirus B19 VP1/VP2 genes (NCBI gene bank). In all patients, acute myocarditis was excluded according to the Dallas classification. All biopsies of 36 controls with no history of myocarditis or recent viral infection were negative for myocardial inflammation and parvovirus B19 genomes. In summary, Parvovirus B19 DNA is present within the myocardium of patients with suspected myocarditis and idiopathic left ventricular dysfunction and can be detected and quantified in endomyocardial specimens via real time PCR.
Authors:
R M Klein; H Jiang; D Niederacher; O Adams; M Du; M Horlitz; P Schley; R Marx; M R Lankisch; M U Brehm; B E Strauer; H E Gabbert; T Scheffold; H Gülker
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Publication Detail:
Type:  Clinical Trial; Journal Article    
Journal Detail:
Title:  Zeitschrift für Kardiologie     Volume:  93     ISSN:  0300-5860     ISO Abbreviation:  Z Kardiol     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-15     Completed Date:  2004-06-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0360430     Medline TA:  Z Kardiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  300-9     Citation Subset:  IM    
Affiliation:
Department of Cardiology, HELIOS Klinikum Wuppertal, Arrenbergerstr. 20, 42117 Wuppertal, Germany. rklein@klinikum-wuppertal.de
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Comorbidity
Endocardium / pathology,  virology
Female
Genome, Viral
Germany / epidemiology
Humans
Male
Middle Aged
Myocarditis / epidemiology*,  pathology,  virology*
Parvoviridae Infections / epidemiology*,  genetics*
Parvovirus B19, Human / genetics*,  isolation & purification*
Polymerase Chain Reaction
Prevalence
Risk Assessment / methods
Risk Factors
Ventricular Dysfunction, Left / embryology*,  pathology,  virology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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