Document Detail


Frequency and differentiation capacity of circulating LTC-IC mobilized by G-CSF or GM-CSF following chemotherapy: a comparison with steady-state bone marrow and peripheral blood.
MedLine Citation:
PMID:  11823040     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The present study was designed to compare directly the frequency of circulating LTC-IC and E-LTC-IC mobilized in peripheral blood (PB) after chemotherapy supported by either G-CSF (PB-G) or GM-CSF (PB-GM) in comparison to steady-state bone marrow (BM) and PB (PB-ST) values in the same patients. MATERIALS AND METHODS: Long-term cultures (LTC) were performed from 20 patients with malignant lymphoma at saturating cell concentrations to assess bulk progenitor cell production and by limiting dilution assay (LDA) to measure both frequency of LTC-IC and their proliferative and differentiation capacities. RESULTS: While CFC production in bulk LTC was higher at weeks 3-5 with PB-G than with PB-GM samples, week-5 LTC-IC and week-10 LTC-IC (E-LTC-IC) frequencies were not different using a LDA. However, the number of CFC derived from a single LTC-IC was higher in PB-G patients than in PB-GM patients (p = 0.01). Interestingly, the frequency of LTC-IC per 1 x 10(5) MNC in mobilized PB positively correlated with one-year marrow progenitor cell recovery, in contrast to the number of autografted CD34(+) cells and CFU-GM per kg. CONCLUSION: Both G-CSF and GM-CSF resulted in similar increases in LTC-IC and E-LTC-IC in PB at comparable levels to those present in BM. However, the differentiation capacity of LTC-IC was higher after mobilization with G-CSF than with GM-CSF, suggesting qualitative differences in LTC-IC mobilized with these growth factors.
Authors:
Lotfi Benboubker; Christian Binet; Guillaume Cartron; Marie-Christine Bernard; Nathalie Clement; Martine Delain; Michel Degenne; Isabelle Desbois; Philippe Colombat; Jorge Domenech
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental hematology     Volume:  30     ISSN:  0301-472X     ISO Abbreviation:  Exp. Hematol.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-02-01     Completed Date:  2002-02-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  74-81     Citation Subset:  IM    
Affiliation:
Laboratory of Hematology, Faculty of Medicine and University Hospital of Tours, Tours, France.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antineoplastic Combined Chemotherapy Protocols / pharmacology
Blood Cells / pathology
Bone Marrow Cells / pathology
Female
Granulocyte Colony-Stimulating Factor / pharmacology*
Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology*
Hematopoietic Stem Cell Mobilization*
Hematopoietic Stem Cells / drug effects,  pathology*
Humans
Male
Middle Aged
Chemical
Reg. No./Substance:
143011-72-7/Granulocyte Colony-Stimulating Factor; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor

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