| Frequency and differentiation capacity of circulating LTC-IC mobilized by G-CSF or GM-CSF following chemotherapy: a comparison with steady-state bone marrow and peripheral blood. | |
MedLine Citation:
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PMID: 11823040 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The present study was designed to compare directly the frequency of circulating LTC-IC and E-LTC-IC mobilized in peripheral blood (PB) after chemotherapy supported by either G-CSF (PB-G) or GM-CSF (PB-GM) in comparison to steady-state bone marrow (BM) and PB (PB-ST) values in the same patients. MATERIALS AND METHODS: Long-term cultures (LTC) were performed from 20 patients with malignant lymphoma at saturating cell concentrations to assess bulk progenitor cell production and by limiting dilution assay (LDA) to measure both frequency of LTC-IC and their proliferative and differentiation capacities. RESULTS: While CFC production in bulk LTC was higher at weeks 3-5 with PB-G than with PB-GM samples, week-5 LTC-IC and week-10 LTC-IC (E-LTC-IC) frequencies were not different using a LDA. However, the number of CFC derived from a single LTC-IC was higher in PB-G patients than in PB-GM patients (p = 0.01). Interestingly, the frequency of LTC-IC per 1 x 10(5) MNC in mobilized PB positively correlated with one-year marrow progenitor cell recovery, in contrast to the number of autografted CD34(+) cells and CFU-GM per kg. CONCLUSION: Both G-CSF and GM-CSF resulted in similar increases in LTC-IC and E-LTC-IC in PB at comparable levels to those present in BM. However, the differentiation capacity of LTC-IC was higher after mobilization with G-CSF than with GM-CSF, suggesting qualitative differences in LTC-IC mobilized with these growth factors. |
Authors:
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Lotfi Benboubker; Christian Binet; Guillaume Cartron; Marie-Christine Bernard; Nathalie Clement; Martine Delain; Michel Degenne; Isabelle Desbois; Philippe Colombat; Jorge Domenech |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental hematology Volume: 30 ISSN: 0301-472X ISO Abbreviation: Exp. Hematol. Publication Date: 2002 Jan |
Date Detail:
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Created Date: 2002-02-01 Completed Date: 2002-02-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0402313 Medline TA: Exp Hematol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 74-81 Citation Subset: IM |
Affiliation:
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Laboratory of Hematology, Faculty of Medicine and University Hospital of Tours, Tours, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antineoplastic Combined Chemotherapy Protocols / pharmacology Blood Cells / pathology Bone Marrow Cells / pathology Female Granulocyte Colony-Stimulating Factor / pharmacology* Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology* Hematopoietic Stem Cell Mobilization* Hematopoietic Stem Cells / drug effects, pathology* Humans Male Middle Aged |
| Chemical | |
Reg. No./Substance:
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143011-72-7/Granulocyte Colony-Stimulating Factor; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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