Document Detail


Frequency-dependent properties of inhibitory synapses in the rostral nucleus of the solitary tract.
MedLine Citation:
PMID:  12522172     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To explore the parameters that define the characteristics of either inhibitory postsynaptic potentials (IPSP) or currents (IPSC) in the gustatory nucleus of the solitary tract (rNST), whole cell patch-clamp recordings were made in horizontal brain stem slices of newborn rats. Neurons were labeled with biocytin to confirm both their location and morphology. IPSPs or IPSCs were evoked by delivering either single, paired-pulse, or tetanic stimulus shocks (0.1-ms duration) via a bipolar stimulating electrode placed on the rNST. Pure IPSP/IPSCs were isolated by the use of glutamate receptor antagonists. For 83% of the single-stimulus-evoked IPSCs, the decay time course was fitted with two exponentials having average time constants of 38 and 181 ms, respectively, while the remainder could be fitted with one exponential of 59 ms. Paired-pulse stimulation resulted in summation of the amplitude of the conditioning and test-stimulus-evoked IPSCs. The decay time course of the test-stimulus-evoked IPSC was slower when compared to the decay time of the conditioning stimulus IPSC. Repeated stimulation resulted in an increase in the decay time of the IPSP/Cs where each consecutive stimulus contributed to prolongation of the decay time constant. Most of the IPSP/Cs resulting from a 1-s >/= 30-Hz tetanic stimulus exhibited an S-shaped decay time course where the amplitude of the IPSP/Cs after termination of the stimulus was initially sustained before starting to decay back to the resting membrane potential. Elevation of extracellular Ca(2+) concentration 10 mM resulted in an increase in the amplitude and decay time of single-stimulus shock-evoked IPSP/Cs. The benzodiazepine GABA(A) receptor modulator diazepam increased the decay time of single-stimulus shock-evoked IPSCs. However, application of diazepam did not affect the decay time of tetanic-stimulation-evoked IPSP/Cs. These results suggest that the decay time of single-stimulus-evoked IPSCs is defined either by receptor kinetics or neurotransmitter clearance from the synaptic cleft or both, while the decay time course of the tetanic stimulus evoked IPSP/Cs is defined by neurotransmitter diffusion from the synaptic cleft. During repetitive stimulation, neurotransmitter accumulates in the synaptic cleft prolonging the decay time constant of the IPSCs. High-frequency stimulation elevates the GABA concentration in the synaptic cleft, which then oversaturates the postsynaptic receptors, and, as a consequence, after termination of the tetanic stimulus, the amplitude of IPSP/Cs is sustained resulting in an S shaped decay time course. This activity-dependent plasticity at GABAergic synapses in the rNST is potentially important in the encoding of taste responses because the dynamic range of stimulus frequencies that result in synaptic plasticity (0-70 Hz) corresponds to the breadth of frequencies that travels via afferent gustatory nerve fibers in response to taste stimuli.
Authors:
Gintautas Grabauskas; Robert M Bradley
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurophysiology     Volume:  89     ISSN:  0022-3077     ISO Abbreviation:  J. Neurophysiol.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2003-01-10     Completed Date:  2003-03-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375404     Medline TA:  J Neurophysiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  199-211     Citation Subset:  IM    
Affiliation:
Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor 48109, USA. gintas@umich.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / pharmacology
Diazepam / pharmacology
Electric Stimulation
Electrophysiology
Evoked Potentials / drug effects,  physiology
GABA Modulators / pharmacology
Neural Inhibition / physiology*
Neurons / physiology
Rats
Rats, Sprague-Dawley
Reaction Time / physiology
Solitary Nucleus / cytology,  physiology*
Synapses / physiology*
Temperature
Grant Support
ID/Acronym/Agency:
DC-00288/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/GABA Modulators; 439-14-5/Diazepam; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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