Document Detail


Frequency-dependent neural activity, CBF, and BOLD fMRI to somatosensory stimuli in isoflurane-anesthetized rats.
MedLine Citation:
PMID:  20350603     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Inhalation anesthetics (e.g. isoflurane) are preferable for longitudinal fMRI experiments in the same animals. We previously implemented isoflurane anesthesia for rodent forepaw stimulation studies, and optimized the stimulus parameters with short stimuli (1-3-s long stimulation with ten electric pulses). These parameters, however, may not be applicable for long periods of stimulation because repetitive stimuli induce neural adaptation. Here we evaluated frequency-dependent responses (pulse width of 1.0 ms and current of 1.5 mA) for 30-s long stimulation under 1.3-1.5% isoflurane anesthesia. The cerebral blood flow (CBF) response (using laser Doppler flowmetry: CBF(LDF)) and field potential (FP) changes were simultaneously measured for nine stimulus frequencies (1-24 Hz). CBF (using arterial spin labeling: CBF(ASL)) and blood oxygenation level dependent (BOLD) fMRI responses were measured at 9.4 T for four stimulus frequencies (1.5-12 Hz). Higher stimulus frequencies (12-24 Hz) produced a larger FP per unit time initially, but decreased more rapidly later due to neural adaptation effects. On the other hand, lower stimulus frequencies (1-3 Hz) induced smaller, but sustained FP activities over the entire stimulus period. Similar frequency-dependencies were observed in CBF(LDF), CBF(ASL) and BOLD responses. A linear relationship between FP and CBF(LDF) was observed for all stimulus frequencies. Stimulation frequency for the maximal cumulative neural and hemodynamic changes is dependent on stimulus duration; 8-12 Hz for short stimulus durations (<10s) and 6-8 Hz for 30-s stimulation. Our findings suggest that neural adaptation should be considered in determining the somatosensory stimulation frequency and duration under isoflurane anesthesia.
Authors:
Tae Kim; Kazuto Masamoto; Mitsuhiro Fukuda; Alberto Vazquez; Seong-Gi Kim
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural     Date:  2010-03-27
Journal Detail:
Title:  NeuroImage     Volume:  52     ISSN:  1095-9572     ISO Abbreviation:  Neuroimage     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-06-10     Completed Date:  2010-09-30     Revised Date:  2014-09-05    
Medline Journal Info:
Nlm Unique ID:  9215515     Medline TA:  Neuroimage     Country:  United States    
Other Details:
Languages:  eng     Pagination:  224-33     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological
Anesthesia
Anesthetics, Inhalation*
Animals
Brain / blood supply,  drug effects,  physiology*
Cerebrovascular Circulation
Electric Stimulation / methods*
Foot / physiology
Forelimb / physiology
Isoflurane*
Linear Models
Magnetic Resonance Imaging
Male
Microelectrodes
Oxygen / blood
Perception* / physiology
Rats
Rats, Sprague-Dawley
Time Factors
Grant Support
ID/Acronym/Agency:
EB003324/EB/NIBIB NIH HHS; EB003375/EB/NIBIB NIH HHS; F32 NS056682/NS/NINDS NIH HHS; NS044589/NS/NINDS NIH HHS; R01 EB003324/EB/NIBIB NIH HHS; R01 EB003324-09/EB/NIBIB NIH HHS; R01 EB003375/EB/NIBIB NIH HHS; R01 EB003375-10/EB/NIBIB NIH HHS; R01 NS044589/NS/NINDS NIH HHS; R01 NS044589-08/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Anesthetics, Inhalation; CYS9AKD70P/Isoflurane; S88TT14065/Oxygen
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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