| Frequency analysis of infrared absorption and vibrational circular dichroism of proteins in D2O solution. | |
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MedLine Citation:
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PMID: 8384041 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The IR absorption frequencies as derived from second derivatives of the Fourier transform IR spectra of the amide I' bands of globular proteins in D2O are compared to those obtained from band fitting of the vibrational circular dichroism (VCD) spectra. The two sets of frequencies are in very good agreement, yielding consistent ranges where amide I' VCD and IR features occur. Use of VCD to complement the IR allows one to add sign information to the frequency information so that features occurring in the overlapping frequency ranges that might arise from different secondary structures can be better discriminated. From this comparison, it is clear that correlation just of the frequency of a given IR transition to secondary structure can lead to a nonunique solution. Different sign patterns were identified for correlated groups of globular proteins in restricted frequency ranges that have been previously assigned to defined secondary structural elements. Hence, different secondary structural elements must contribute band components to a given frequency range. |
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Authors:
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P Pancoska; L Wang; T A Keiderling |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Protein science : a publication of the Protein Society Volume: 2 ISSN: 0961-8368 ISO Abbreviation: Protein Sci. Publication Date: 1993 Mar |
Date Detail:
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Created Date: 1993-04-22 Completed Date: 1993-04-22 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9211750 Medline TA: Protein Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 411-9 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Illinois, Chicago 60680. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Circular Dichroism Deuterium Deuterium Oxide Fourier Analysis Molecular Structure Protein Structure, Secondary Proteins / chemistry* Solutions Spectrophotometry, Infrared Vibration Water |
| Grant Support | |
ID/Acronym/Agency:
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GM 30147/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Proteins; 0/Solutions; 7732-18-5/Water; 7782-39-0/Deuterium; 7789-20-0/Deuterium Oxide |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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