Document Detail


Frequency and Expression of Inhibitory Markers of CD4+CD25+ FOXP3+ Regulatory T Cells in Patients with Common Variable Immunodeficiency.
MedLine Citation:
PMID:  23432692     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Common variable immunodeficiency (CVID) is the most symptomatic primary antibody deficiency associated with recurrent infections and chronic inflammatory diseases as well as autoimmunity. CD4(+) CD25(+) FOXP3(+) regulatory T cells (Tregs) are critical T cell subsets for maintaining self-tolerance and regulation of immune response to antigens thus play a pivotal role in preventing autoimmunity. Thirty seven CVID patients and 18 age-sex matched controls were enrolled. Peripheral blood mononuclear cells (PBMCs) were obtained from both groups and the percentage of Tregs was calculated using flow cytometry method. The mRNA expression of Tregs surface markers cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) and glucocorticoid induced tumor necrosis factor receptor (GITR) which are associated with Tregs inhibitory function were compared between patients and controls by quantitative Real-time PCR TaqMan method. The results revealed that the frequency of Tregs was significantly lower in CVID patients than normal individuals (P<0.001). In addition, CVID patients with autoimmunity were found to have markedly reduced proportion of Tregs compared to those cases without autoimmune diseases (P=0.023). A significant difference was seen in FOXP3 expression between CVID patients and controls (P<0.001). The mRNAs of CTLA-4 and GITR genes were expressed at lower levels in CVID patients compared to control group (P=0.005 and <0.001, respectively). Our findings showed reduced proportion of Tregs in CVID patients together with down-regulation of FOXP3 protein and diminished expression of inhibitory Tregs' markers. It could be concluded that all of these changes may be responsible for cellular immune dysregulation observed in these patients especially those with autoimmune manifestation.
Authors:
Nargess Arandi; Abbas Mirshafiey; Hassan Abolhassani; Mahmood Jeddi-Tehrani; Rozita Edalat; Bamdad Sadeghi; Mohammadreza Shaghaghi; Asghar Aghamohammadi
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-23
Journal Detail:
Title:  Scandinavian journal of immunology     Volume:  -     ISSN:  1365-3083     ISO Abbreviation:  Scand. J. Immunol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0323767     Medline TA:  Scand J Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors. Scandinavian Journal of Immunology © 2013 Blackwell Publishing Ltd.
Affiliation:
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
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