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Frequencies of mitogen-reactive B cells in the mouse. II. Frequencies of B cells producing antibodies which lyse sheep or horse erythrocytes, and trinitrophenylated or nitroiodophenylated sheep erythrocytes.
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MedLine Citation:
PMID:  325169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The B-cell mitogens LPS and lipoprotein stimulate 20-35 percent of all B cells in the spleen of 6- to 8-wk old C3H/Tif mice, as determined by limiting dilution analysis of precursors. Each reactive cell grows to a clone of IgM-secreting PFC, enumerated in a hemolytic plaque assay detecting all IgM secreting cells, regardless of v-region specificity. We have used these mitogens to reveal the total repertoire of Ig specificities produced by these mitogen-reactive B cells. We have determined in plaque assays with six different target erythrocytes the number of spleen cells limiting to one the number of mitogen-reactive B cells detected as specific IgM-secreting clones in each of these plaque assays. By this method, the absolute frequencies of precursor B cells with defined v-gene specificities could be calculated, for at least, one third of all B cells. The frequencies of specific IgM-plaque-forming B-cell clones within the total pool of mitogen-reactive B cells was 1 in 10 for NIP(12),-SRC, 1 in 50 for TNP(12)- SRC, 1 in 100 for NIP(1)-SRC, 1 in 160 for TNP(3)- SRC, 1 in 500 for HRC, and 1 in 1,000 for SRC. These frequencies were the same in the LPS- and in the lipoprotein-reactive B-cell population for TNP(30)- SRC and SRC.
Authors:
J Andersson; A Coutinho; F Melchers
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  145     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1977 Jun 
Date Detail:
Created Date:  1977-07-18     Completed Date:  1977-07-18     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1520-30     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibody Specificity
Antibody-Producing Cells
B-Lymphocytes / immunology*
Complement System Proteins
Erythrocytes / drug effects,  immunology
Hemolytic Plaque Technique
Horses / immunology
Immunoglobulin M
Iodobenzenes / pharmacology
Lipopolysaccharides / pharmacology
Lipoproteins / pharmacology
Lymphocyte Activation*
Mice
Mice, Inbred C3H
Mitogens / pharmacology*
Polysaccharides, Bacterial / pharmacology
Rats
Rats, Inbred Lew
Sheep / immunology
Spleen / cytology,  immunology
T-Lymphocytes / immunology
Trinitrobenzenes / pharmacology
Chemical
Reg. No./Substance:
0/Immunoglobulin M; 0/Iodobenzenes; 0/Lipopolysaccharides; 0/Lipoproteins; 0/Mitogens; 0/Polysaccharides, Bacterial; 0/Trinitrobenzenes; 9007-36-7/Complement System Proteins
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 6 Year: 1977
Volume: 145 Issue: 6
First Page: 1520 Last Page: 1530
ID: 2180691
Publisher Id: 14561520
PubMed Id: 325169

Frequencies of mitogen-reactive B cells in the mouse. II. Frequencies of B cells producing antibodies which lyse sheep or horse erythrocytes, and trinitrophenylated or nitrodophenylated sheep erythrocytes
J Andersson
A Coutinho
F Melchers


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