Document Detail

Freezing and recovery of hybridoma cell lines.
MedLine Citation:
PMID:  18265081     Owner:  NLM     Status:  MEDLINE    
Liquid nitrogen storage is the method of choice for long-term safekeeping of hybridoma cell lines. Frozen aliquots of originally isolated hybridomas provide insurance against loss of antibody production and vigor during culture. There are many variations of cell freezing methods in use. However, for freezing and recovering hybridomas and lymphoid cells in general, the protocol described in this unit is simple and has been successful.
S A Fuller; M Takahashi; J G Hurrell
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]     Volume:  Chapter 11     ISSN:  1934-3647     ISO Abbreviation:  Curr Protoc Mol Biol     Publication Date:  2001 May 
Date Detail:
Created Date:  2008-02-11     Completed Date:  2008-02-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8908160     Medline TA:  Curr Protoc Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit11.9     Citation Subset:  IM    
Allelix Inc., Mississauga, Ontario, Canada.
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MeSH Terms
Antibodies, Monoclonal / isolation & purification*
Cell Line
Cryopreservation / methods*
Hybridomas / immunology*
Reg. No./Substance:
0/Antibodies, Monoclonal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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