| Freezing preservation of the mammalian cardiac explant. V. Cryoprotection by ethanol. | |
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MedLine Citation:
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PMID: 1395684 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We studied the colligative cryoprotective effect of ethanol (EtOH) in preserving the isolated rat heart frozen at -3.4 degrees C or unfrozen at -1.4 degrees C. Addition of 4.7% (v/v) EtOH to a cardioplegic solution, CP-14, raised the osmolality from 280 to 1100 mOsm/kg H2O and lowered the melting point from -0.52 to -2.1 degrees C. Freezing of the cardiac explant at -3.4 degrees C for 6 h resulted in 34.3 +/- 1.9% of the tissue water as ice; recovery of cardiac output (CO) was 50%. Polyethylene glycol, which at 5% (w/v) has been shown to cryoprotect the hearts during freezing at -1.4 degrees C, did not improve the protective effect of 4.7% EtOH. CP-14 + 4.7% EtOH did not freeze at -1.4 degrees C. After 6 h storage, CO in hearts flushed with CP-14 + 4.7% EtOH oxygenated with 95% O2/5%CO2 returned to almost control level and was much higher than that in hearts flushed with 100% O2 saturated-CP-14 + 4.7% EtOH. Storage of 8 and 12 h reduced CO to 87 +/- 9 and 60 +/- 5% of control. By employing EtOH as a colligative cryoprotectant, we preserved the adult mammalian heart frozen at -3.4 degrees C or unfrozen at -1.4 degrees C, suggesting that this small molecular weight, penetrating substance may be a suitable cryoprotectant for long-term storage of the cardiac explant at high subzero temperatures. |
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Authors:
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T Wang; M C Banker; M Claydon; G L Hicks; J R Layne |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cryobiology Volume: 29 ISSN: 0011-2240 ISO Abbreviation: Cryobiology Publication Date: 1992 Aug |
Date Detail:
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Created Date: 1992-11-13 Completed Date: 1992-11-13 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0006252 Medline TA: Cryobiology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 470-7 Citation Subset: IM |
Affiliation:
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Department of Surgery, University of Rochester, New York 14642. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cardiac Output Cryopreservation / methods* Ethanol* / toxicity Evaluation Studies as Topic Heart* / drug effects, physiology Ice Male Osmolar Concentration Polyethylene Glycols Rats Rats, Sprague-Dawley Temperature Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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HL45129/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ice; 0/Polyethylene Glycols; 64-17-5/Ethanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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