Document Detail

Freezing monolayers of cells without gap junctions.
MedLine Citation:
PMID:  12686210     Owner:  NLM     Status:  MEDLINE    
Cells in monolayers have been reported to be more susceptible to freezing injury than the same cell type frozen in dispersed suspensions. There appears to be an enhanced susceptibility to intracellular freezing in the monolayers, which is thought to be facilitated by the presence of gap junctions allowing the spread of ice between neighbouring cells. MDCK Type II cells do not form gap junctions in monolayer culture. When frozen at rates of 0.2 to 10 degrees C/min, monolayers in 10% (v/v) propane-1,2-diol or dimethyl sulphoxide showed little influence of cooling rate on survival. This suggested that, in the absence of gap junctions, cells in monolayers did not display enhanced susceptibility to intracellular freezing. In contrast, however, monolayers frozen in glycerol showed a marked increase in cell damage when cooled at rates higher than 0.5 degrees C/min. This does not necessarily counter the suggestion that lack of gap junctions mitigates intracellular freezing as there is evidence that glycerol may itself promote intracellular freezing.
W J Armitage; B K Juss
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryobiology     Volume:  46     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-10     Completed Date:  2003-12-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  194-6     Citation Subset:  IM    
Division of Ophthalmology, Bristol Eye Hospital, University of Bristol, Lower Maudlin Street, BS1 2LX, Bristol, United Kingdom.
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MeSH Terms
Cell Culture Techniques / methods
Cell Line
Cell Survival / drug effects,  physiology
Cryoprotective Agents / pharmacology*
Dimethyl Sulfoxide / pharmacology
Epithelial Cells / drug effects,  physiology*
Gap Junctions / physiology*
Glycerol / pharmacology
Propylene Glycol / pharmacology
Reg. No./Substance:
0/Cryoprotective Agents; 56-81-5/Glycerol; 57-55-6/Propylene Glycol; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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