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Freezing and Freeze-Drying of the Bacterium Rahnella aquatilis BNM 0523: Study of Protecting Agents, Rehydration Media and Freezing Temperatures.
MedLine Citation:
PMID:  21913947     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims:  The aim of the present study was to evaluate and compare freezing and freeze-drying treatments for conserving Rahnella aquatilis (BNM 0523) with the goal to achieve an adequate commercial formulation of this biocontrol agent. Methods and Results:  The effect of several protective agents, rehydration media and freezing temperatures, on the viability and functional activity of the R. aquatilis was investigated. The storage stability at 3 months and 4 years was determined by checking the viability of the cells and their biocontrol capability against Botrytis cinerea by measuring the percentage of reduction of disease severity on apple. The best results were obtained by the freeze-drying of the cells using a mixture of skimmed non fat milk 10%; yeast extract 0.5%; and glucose 1% (SMYG) as the protecting and rehydrating medium, and a quickly freezing (-70°C) before the freeze-drying. In this case the viability of the cells after 4 years was 98% and their antagonistic ability showed a little decrease with respect fresh cells. Conclusions:  The studies showed that R. aquatilis was resistant to freezing and freeze-drying when it was used a mixture of cryoprotectants, and that it was possible to obtain inoculums with high viability and good effectiveness for reduction of decay caused by Botrytis cinerea.
Authors:
Leonardo G Navarta; Juan Calvo; Viviana Calvente; Delia Benuzzi; María Isabel Sanz
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-13
Journal Detail:
Title:  Letters in applied microbiology     Volume:  -     ISSN:  1472-765X     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 The Society for Applied Microbiology.
Affiliation:
Area de Tecnología Química y Biotecnología. Facultad de Química Bioquímica y Farmacia. Universidad Nacional de San Luis. 5700 San Luis. Argentina.
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