| Freeze-thawing as the factor of spontaneous activation of spermatozoa motility in common carp (Cyprinus carpio L.). | |
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MedLine Citation:
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PMID: 19723519 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present study, we investigated the possibility of spontaneous carp spermatozoa activation by freeze-thawing. To evaluate this, the parameters of spermatozoa motility percentage, velocity, ATP content level and fertility rate of sperm were used. The motility and velocity of spermatozoa activated by freeze-thawing were characterized by motile spermatozoa with a median value of 16% and a velocity of 98 microm/s. In addition, the motility and velocity of sperm from the thawed samples were significantly lower than in the control (median value of 100% for sperm motility and 175 microm/s for sperm velocity). Furthermore, a spontaneously activated spermatozoa motility terminated within five minutes post-thaw time. After freeze-thawing the ATP level significantly decreased with post-thaw time (46 nmol ATP/10(9) and 10 nmol ATP/10(9) at 25s and 10 min after thawing, respectively). Fertility of spermatozoa was not significantly affected within 10 min post-thaw. On the other hand, the fertility of frozen-thawed sperm was significantly lower if compared to fresh sperm. We conclude that the freeze-thawing procedure spontaneously activated spermatozoa motility in common carp. However, this activation did not negatively affect the fertility of frozen-thawed sperm. |
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Authors:
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S Boryshpolets; B Dzyuba; M Rodina; P Li; M Hulak; D Gela; O Linhart |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-31 |
Journal Detail:
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Title: Cryobiology Volume: 59 ISSN: 1090-2392 ISO Abbreviation: Cryobiology Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-11-30 Completed Date: 2010-02-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0006252 Medline TA: Cryobiology Country: United States |
Other Details:
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Languages: eng Pagination: 291-6 Citation Subset: IM |
Affiliation:
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University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, 389 25 Vodnany, Czech Republic. boryss00@vurh.jcu.cz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate Animals Carps Cryopreservation / methods*, veterinary* Freezing Male Semen Preservation / methods*, veterinary* Sperm Motility / physiology* Spermatozoa / physiology* |
| Chemical | |
Reg. No./Substance:
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56-65-5/Adenosine Triphosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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