Document Detail

[Freeze grinding combined ultrasonic technique for the treatment of nail test material].
MedLine Citation:
PMID:  23213783     Owner:  NLM     Status:  In-Process    
OBJECTIVE: To investigate the feasibility of the new method of combining freeze grinding with ultrasonic technique for the pretreatment of the nail for toxicological and pharmaceutical analysis and to compare the advantages and disadvantages of this method with other traditional methods.
METHODS: Five pretreatment methods were examined. Scanning electron microscope (SEM) was used to observe the microstructural changes of the nail.
RESULTS: The microscopic structure of nail totally destroyed after alkali treatment. The hierarchy mode of the internal structure became obvious and tight after acid hydrolysis, which became indistinct after methanol infiltration. The structure of nail broke to pieces after ultrasonic technique. After freeze grinding combined ultrasonic technique, the particle structure kept original shape, and its size was one hundred times smaller than which after manual way.
CONCLUSION: The freeze grinding combined ultrasonic technique can improve the release efficiency, and ensure the stability of the toxicant or drug during the pretreatment process. It is appropriate for toxicological and pharmaceutical analysis in the nail.
Hang Chen; Ping Xiang; Qi-ran Sun; Min Shen
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Fa yi xue za zhi     Volume:  28     ISSN:  1004-5619     ISO Abbreviation:  Fa Yi Xue Za Zhi     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-12-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426151     Medline TA:  Fa Yi Xue Za Zhi     Country:  China    
Other Details:
Languages:  chi     Pagination:  342-6     Citation Subset:  IM    
Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
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