Document Detail


Freeze fracture studies of human neuromuscular junctions. Membrane alterations observed in myasthenia gravis.
MedLine Citation:
PMID:  7230737     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Freeze fracture replicas of neuromuscular junctions from normal human patients and those with facioscapulohumeral and limb girdle muscular dystrophies, progressive muscular atrophy, and myasthenia gravis were examined by stereoscopic electron microscopy. Endplates from most human patients closely resemble those of normal adult rats fixed by intravascular perfusion. However, endplates from patients with myasthenia gravis have clinically relevant alterations in postsynaptic membrane infrastructure, including decreased number of P-face particles and increased number of E-face particles in the crests of the junctional folds. Other junctional fold crests are narrowed or obliterated and are replaced by incompletely sealed membrane vesicles rich in intramembrane particles. Similar alterations are not produced in rat endplates by immersion fixation, exposure to low pH, brief hypoxia, prolonged or extreme hypothermia, prolonged or high frequency nerve stimulation, or by prolonged nerve quiescence. Rather, the destructive alterations observed in junctional fold membranes in myasthenia gravis endplates are correlated with decreased acetylcholine sensitivity and to removal of endplate acetylcholine receptors by immunologic processes.
Authors:
J E Rash; C S Hudson; W F Graham; R F Mayer; J E Warnick; E X Albuquerque
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Laboratory investigation; a journal of technical methods and pathology     Volume:  44     ISSN:  0023-6837     ISO Abbreviation:  Lab. Invest.     Publication Date:  1981 Jun 
Date Detail:
Created Date:  1981-07-20     Completed Date:  1981-07-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376617     Medline TA:  Lab Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  519-30     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biopsy
Freeze Fracturing*
Humans
Membrane Lipids / analysis
Membrane Proteins / analysis
Microscopy, Electron
Myasthenia Gravis / pathology*
Neuromuscular Junction / pathology*
Rats
Receptors, Cholinergic / metabolism
Grant Support
ID/Acronym/Agency:
NS06779/NS/NINDS NIH HHS; NS14648/NS/NINDS NIH HHS; NS15991/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Membrane Lipids; 0/Membrane Proteins; 0/Receptors, Cholinergic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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