Document Detail


Freeze-drying as a preserving preparation technique for in vitro testing of human skin.
MedLine Citation:
PMID:  23278895     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
In vitro testing of drugs with excised human skin is a valuable prerequisite for clinical studies. However, the analysis of excised human skin presents several obstacles. Ongoing drug diffusion, microbial growth and changes in hydration state influence the results of drug penetration studies. In this work, we evaluate freeze-drying as a preserving preparation method for skin samples to overcome these obstacles. We analyse excised human skin before and after freeze-drying and compare these results with human skin in vivo. Based on comprehensive thermal and spectroscopic analysis, we demonstrate comparability to in vivo conditions and exclude significant changes within the skin samples due to freeze-drying. Furthermore, we show that freeze-drying after skin incubation with drugs prevents growth of drug crystals on the skin surface due to drying effects. In conclusion, we introduce freeze-drying as a preserving preparation technique for in vitro testing of human skin.
Authors:
Lutz Franzen; Lucie Vidlářová; Karl-Heinz Kostka; Ulrich F Schaefer; Maike Windbergs
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Publication Detail:
Type:  Letter    
Journal Detail:
Title:  Experimental dermatology     Volume:  22     ISSN:  1600-0625     ISO Abbreviation:  Exp. Dermatol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  54-6     Citation Subset:  IM    
Copyright Information:
© 2013 John Wiley & Sons A/S.
Affiliation:
Department of Biopharmaceutics and Pharmaceutical Technology, Saarland University, Saarbruecken, Germany.
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