| FREEZE-DRIED HEART VALVE SCAFFOLDS. | |
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MedLine Citation:
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PMID: 22224705 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In this study, structure and biomechanical properties of freeze-dried decellularized porcine pulmonary heart valves were investigated. Heart valves were dissected from porcine hearts. The tissues were decellularized and separated in three groups: (1) without lyoprotectant, (2) with 5% sucrose and (3) with a mixture of 2.5% sucrose and 2.5% hydroxyl ethylene starch (HES), and then underwent freeze-drying. Freeze-drying in the absence of lyoprotectants caused an overall more disintegrated appearance of the histological architecture of the porcine valves, especially between the fibrosa and the ventricularis layers. Freeze-dried tissue with lyoprotectants have a looser network of collagen and elastic fibers with bigger pore sizes. Tissue freeze-dried in the absence of lyoprotecants had the largest pore sizes, whereas the tissue freeze-dried in the presence of protectants showed pores of intermediate sizes between the decellularized tissue and the unprotected freeze-dried samples. Tissue freeze-dried with sucrose alone displayed less porosity compared to tissue freeze-dried with the sucrose/HES mixture, whereas no significant differences in biomechanical properties were observed. Decellularization decreased the elastic modulus of artery tissue. The elastic modulus of freeze-dried tissue without protectants resembled that of decellularized tissue. The elastic modulus values of freeze-dried tissue stabilized by lyoprotectants were greater compared to those of decellularized tissue, but similar to those of native tissue. |
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Authors:
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Shangping Wang; Tobias Goecke; Carsten Meixner; Axel Haverich; Andres Hilfiker; Willem Frederik Wolkers |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-7 |
Journal Detail:
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Title: Tissue engineering. Part C, Methods Volume: - ISSN: 1937-3392 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101466663 Medline TA: Tissue Eng Part C Methods Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Leibniz Universität Hannover, Germany, Institute of Multiphase Processes, Hannover, Germany; wang@imp.uni-hannover.de. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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