Document Detail

Free sterols of the cerebral white matter in experimental severe hypoxia.
MedLine Citation:
PMID:  9377076     Owner:  NLM     Status:  MEDLINE    
Wistar rats, aged 3.5 months, were subjected to severe hypoxia by placing them for 3 minutes in an atmosphere containing 2 kPa of oxygen. The myelin fraction was isolated from brains of the experimental animals at the following periods after the hypoxia event: 4 and 24 hours, 14 days, 2 months. The myelin neutral lipid extracts were fed into GC-MS system (gas chromatography-mass spectrometry) for identification of the separated free sterols. The free sterol spectrum of the myelin fraction of rats following the experimental hypoxia was variable unstable. The content of free cholesterol decreased and the contribution of minor sterols (24-ethylcholesterol, 24-ethyl-4-cholesten-3-on, 22-propyl-3 beta-hydroxy-5,24-cholestadien) changed. Lanosterol, one of the natural precursors in cholesterol biosynthesis happened to appear, and the content of the other precursor, desmosterol-increased. These changes may be indicative of some disturbances in the process of cholesterol biosynthesis induced by the experimental hypoxia. The occurrence of ethyl- and propylderivatives of cholesterol (compound found mainly in plant sterol spectra) in both the control and experimental myelin samples in either of the examined post-hypoxia periods is most likely the result of incorporation into myelin lipids of ingested plant sterols, nevertheless, endogenous synthesis cannot be ruled out.
J Dorszewska; Z Adamczewska-Goncerzewicz
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Folia neuropathologica / Association of Polish Neuropathologists and Medical Research Centre, Polish Academy of Sciences     Volume:  35     ISSN:  1641-4640     ISO Abbreviation:  Folia Neuropathol     Publication Date:  1997  
Date Detail:
Created Date:  1997-11-10     Completed Date:  1997-11-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9437431     Medline TA:  Folia Neuropathol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  115-20     Citation Subset:  IM    
Department of Neurology, Karol Marcinkowski University of Medical Sciences, Poznan.
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MeSH Terms
Anoxia / diagnosis*
Cerebral Cortex / chemistry*
Chromatography, Gas
Mass Spectrometry
Rats, Wistar
Sterols / analysis*
Reg. No./Substance:

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