| Free radicals upregulate complement expression in rabbit isolated heart. | |
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MedLine Citation:
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PMID: 10899056 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Both free radicals and complement activation can injure tissue. Our study determined whether free radicals alter complement production by the myocardium. Isolated hearts from New Zealand White rabbits were perfused on a Langendorff apparatus and exposed to xanthine (X; 100 microM) plus xanthine oxidase (XO; 8 mU/ml) (X/XO). The free radical-generating system significantly (P < 0.05) increased C1q and also increased C1r, C3, C8, and C9 transcription compared with controls. Immunohistological examination revealed augmented membrane attack complex deposition on X/XO-treated tissue. X/XO-treated hearts also exhibited significant (P < 0.05) increases in coronary perfusion pressure and left ventricular end-diastolic pressure and a decrease in left-ventricular developed pressure. N-(2-mercaptopropionyl)-glycine (3 mM), in conjunction with the superoxide dismutase mimetic SC-52608 (100 microM), significantly (P < 0.05) reduced the upregulation of C1q, C1r, C3, C8, and C9 mRNA expression elicited by X/XO. The antioxidants also ameliorated the deterioration in function caused by X/XO. Local complement activation may represent a mechanism by which free radicals mediate tissue injury. |
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Authors:
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E J Tanhehco; K Yasojima; P L McGeer; R A Washington; B R Lucchesi |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 279 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2000-08-16 Completed Date: 2000-08-16 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: H195-201 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Complement C1q / genetics Complement C3 / genetics Complement C8 / genetics Complement C9 / genetics Complement System Proteins / genetics* Free Radicals / pharmacology Gene Expression Regulation / drug effects* Heart / drug effects* Male Myocardium / metabolism* Rabbits Receptors, Complement / genetics Transcription, Genetic Xanthine / pharmacology Xanthine Oxidase / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Complement C3; 0/Complement C8; 0/Complement C9; 0/Free Radicals; 0/Receptors, Complement; 69-89-6/Xanthine; 80295-33-6/Complement C1q; 9007-36-7/Complement System Proteins; EC 1.17.3.2/Xanthine Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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