Document Detail

Free radical metabolite of uric acid.
MedLine Citation:
PMID:  2828349     Owner:  NLM     Status:  MEDLINE    
Uric acid has previously been shown to act as a water-soluble antioxidant. Although the antioxidant activity of uric acid has been attributed to its ability to scavenge free radicals, the one-electron uric acid oxidation product of such a scavenging reaction has not been detected. It order to determine whether a free radical metabolite of uric acid could be formed via one-electron redox processes, we oxidized uric acid with potassium permanganate, horseradish peroxidase/hydrogen peroxide, and hematin/hydrogen peroxide systems. With the use of the rapid-mixing, continuous-flow electron spin resonance technique, we were able to detect the urate anion free radical in all three radical-generating systems. Based on N15-isotopic-labeling experiments, we show that the unpaired electron of this radical is located primarily on the five-membered ring of the purine structure. We were also able to demonstrate that this radical could be scavenged by ascorbic acid.
K R Maples; R P Mason
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  263     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1988 Feb 
Date Detail:
Created Date:  1988-03-07     Completed Date:  1988-03-07     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1709-12     Citation Subset:  IM    
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
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MeSH Terms
Ascorbic Acid
Electron Spin Resonance Spectroscopy
Free Radicals
Horseradish Peroxidase
Hydrogen Peroxide
Potassium Permanganate
Uric Acid / metabolism*
Reg. No./Substance:
0/Free Radicals; 16009-13-5/Hemin; 50-81-7/Ascorbic Acid; 69-93-2/Uric Acid; 7722-64-7/Potassium Permanganate; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.-/Horseradish Peroxidase

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