| Free radical-mediated membrane depolarization in renal and cardiac cells. | |
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MedLine Citation:
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PMID: 3567194 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell membrane potential was measured with a flow cytometer by quantitating the intracellular accumulation of a fluorescent cationic carbocyanine dye. We used this system to demonstrate depolarization upon the addition of hydrogen peroxide (10-1,000 microM) and ferrous chloride (25-100 microM) to cultures of either neonatal rat myocardial or LLC-PK1 renal epithelial cells. Ferrous chloride-induced depolarization was prevented by superoxide dismutase, catalase and dimethyl sulfoxide, suggesting roles for the superoxide anion, hydrogen peroxide and the hydroxyl radical in effecting this depolarization, possibly through a Fenton-type reaction mechanism. Supplementation of either cell type with 2 microM tocopherol acid succinate during growth in tissue culture, prior to exposure to the oxidizing agent, decreased the magnitude of the depolarization in both cell types. The results are consistent with a role for tocopherols in scavenging free radical species responsible for the depolarization of the cell membrane. |
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Authors:
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J A Scott; A J Fischman; B A Khaw; C J Homcy; C A Rabito |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 899 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1987 May |
Date Detail:
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Created Date: 1987-06-17 Completed Date: 1987-06-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 76-82 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Cell Line Cell Membrane / drug effects, physiology* Ferrous Compounds / pharmacology* Free Radicals Heart / physiology* Hydrogen Peroxide / pharmacology* Kidney / physiology Membrane Potentials / drug effects Rats |
| Grant Support | |
ID/Acronym/Agency:
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AM 27401/AM/NIADDK NIH HHS; CA33570/CA/NCI NIH HHS; HL-56212/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ferrous Compounds; 0/Free Radicals; 7722-84-1/Hydrogen Peroxide; 7758-94-3/ferrous chloride |
| Comments/Corrections | |
Erratum In:
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Biochim Biophys Acta 1987 Sep 3;902(3):409 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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