Document Detail

Free radical-mediated membrane depolarization in renal and cardiac cells.
MedLine Citation:
PMID:  3567194     Owner:  NLM     Status:  MEDLINE    
Cell membrane potential was measured with a flow cytometer by quantitating the intracellular accumulation of a fluorescent cationic carbocyanine dye. We used this system to demonstrate depolarization upon the addition of hydrogen peroxide (10-1,000 microM) and ferrous chloride (25-100 microM) to cultures of either neonatal rat myocardial or LLC-PK1 renal epithelial cells. Ferrous chloride-induced depolarization was prevented by superoxide dismutase, catalase and dimethyl sulfoxide, suggesting roles for the superoxide anion, hydrogen peroxide and the hydroxyl radical in effecting this depolarization, possibly through a Fenton-type reaction mechanism. Supplementation of either cell type with 2 microM tocopherol acid succinate during growth in tissue culture, prior to exposure to the oxidizing agent, decreased the magnitude of the depolarization in both cell types. The results are consistent with a role for tocopherols in scavenging free radical species responsible for the depolarization of the cell membrane.
J A Scott; A J Fischman; B A Khaw; C J Homcy; C A Rabito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  899     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1987 May 
Date Detail:
Created Date:  1987-06-17     Completed Date:  1987-06-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  76-82     Citation Subset:  IM    
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MeSH Terms
Animals, Newborn
Cell Line
Cell Membrane / drug effects,  physiology*
Ferrous Compounds / pharmacology*
Free Radicals
Heart / physiology*
Hydrogen Peroxide / pharmacology*
Kidney / physiology
Membrane Potentials / drug effects
Grant Support
Reg. No./Substance:
0/Ferrous Compounds; 0/Free Radicals; 7722-84-1/Hydrogen Peroxide; 7758-94-3/ferrous chloride
Erratum In:
Biochim Biophys Acta 1987 Sep 3;902(3):409

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