Document Detail


Free radical-initiated and gap junction-mediated bystander effect due to nonuniform distribution of incorporated radioactivity in a three-dimensional tissue culture model.
MedLine Citation:
PMID:  11175669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate the biological effects of nonuniform distribution of radioactivity in mammalian cells, we have developed a novel three-dimensional tissue culture model. Chinese hamster V79 cells were labeled with tritiated thymidine and mixed with unlabeled cells, and multicellular clusters (approximately 1.6 mm in diameter) were formed by gentle centrifugation. The short-range beta particles emitted by (3)H impart only self-irradiation of labeled cells without significant cross-irradiation of unlabeled bystander cells. The clusters were assembled in the absence or presence of 10% dimethyl sulfoxide (DMSO) and/or 100 microM lindane. DMSO is a hydroxyl radical scavenger, whereas lindane is an inhibitor of gap junctional intercellular communication. The clusters were maintained at 10.5 degrees C for 72 h to allow (3)H decays to accumulate and then dismantled, and the cells were plated for colony formation. When 100% of the cells were labeled, the surviving fraction was exponentially dependent on the mean level of radioactivity per labeled cell. A two-component exponential response was observed when either 50 or 10% of the cells were labeled. Though both DMSO and lindane significantly protected the unlabeled or bystander cells when 50 or 10% of the cells were labeled, the effect of lindane was greater than that of DMSO. In both cases, the combined treatment (DMSO + lindane) elicited maximum protection of the bystander cells. These results suggest that the bystander effects caused by nonuniform distributions of radioactivity are affected by the fraction of cells that are labeled. Furthermore, at least a part of these bystander effects are initiated by free radicals and are likely to be mediated by gap junctional intercellular communication.
Authors:
A Bishayee; H Z Hill; D Stein; D V Rao; R W Howell
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Radiation research     Volume:  155     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-03-29     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  335-44     Citation Subset:  IM; S    
Affiliation:
Division of Radiation Research, Department of Radiology, University of Medicine and Dentistry of New Jersey, 07103, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Beta Particles
Biological Transport
Cell Communication
Cricetinae
Cricetulus
Culture Techniques / methods*
Dimethyl Sulfoxide / pharmacology
Fibroblasts / metabolism,  radiation effects*,  ultrastructure
Free Radicals
Gap Junctions / physiology*
Lindane / pharmacology
Radiation-Protective Agents / pharmacology
Thymidine / metabolism*
Tritium / metabolism*
Grant Support
ID/Acronym/Agency:
1 S10 RR14753-01/RR/NCRR NIH HHS; R01 CA083838-01A1/CA/NCI NIH HHS; R01 CA83838/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Free Radicals; 0/Radiation-Protective Agents; 10028-17-8/Tritium; 50-89-5/Thymidine; 58-89-9/Lindane; 67-68-5/Dimethyl Sulfoxide
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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