Document Detail


Free radical formation after intensive exercise in thoroughbred skeletal muscles.
MedLine Citation:
PMID:  24833984     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Although high oxygen consumption in skeletal muscle may result in severe oxidative stress, there are no direct studies that have documented free radical production in horse muscles after intensive exercise. To find a new parameter indicating the muscle adaptation state for the training of Thoroughbred horses, we examined free radical formation in the muscle by using electron paramagnetic resonance (EPR). Ten male Thoroughbred horses received conventional training for 18 weeks. Before and after the training period, all horses performed an exhaustive incremental load exercise on a 6% incline treadmill. Muscle samples of the middle gluteal muscle were taken pre-exercise and 1 min, 1 hr, and 1 day after exercise. Muscle fiber type composition was also determined in the pre-exercise samples by immunohistochemical staining with monoclonal antibody to myosin heavy chain. We measured the free radical in the muscle homogenate using EPR at room temperature, and the amount was expressed as relative EPR signal intensity. There was a significant increase in Type IIA muscle fiber composition and a decrease in Type IIX fiber composition after the training period. Before the training period, the mean value of the relative EPR signal intensity showed a significant increase over the pre-exercise value at 1 min after the exercise and an incomplete recovery at 24 hr after the exercise. While no significant changes were found in the relative EPR signal intensity after the training period. There was a significant relationship between percentages of Type IIA fiber and change rates in EPR signal intensity at 1 min after exercise. The measurement of free radicals may be useful for determining the muscle adaptation state in the training of Thoroughbred horses.
Authors:
Yoshio Minami; Minako Kawai; Taiko C Migita; Atsushi Hiraga; Hirofumi Miyata
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Publication Detail:
Type:  Journal Article     Date:  2011-07-20
Journal Detail:
Title:  Journal of equine science / Japanese Society of Equine Science     Volume:  22     ISSN:  1340-3516     ISO Abbreviation:  J Equine Sci     Publication Date:  2011  
Date Detail:
Created Date:  2014-05-16     Completed Date:  2014-05-16     Revised Date:  2014-05-23    
Medline Journal Info:
Nlm Unique ID:  9503751     Medline TA:  J Equine Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  21-8     Citation Subset:  -    
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