Document Detail

Free-radical degradation of high-molar-mass hyaluronan induced by Weissberger's oxidative system: Potential antioxidative effect of bucillamine.
MedLine Citation:
PMID:  23353859     Owner:  NLM     Status:  Publisher    
OBJECTIVES: Hyaluronan (HA), one of the main components of extracellular matrix, is a glycosaminoglycan composed of repeating disaccharide units of N-acetyl-d-glucosamine and d-glucuronic acid linked by β-(1→4) and β-(1→3) glycoside bonds. High-molar-mass HA was used as a model for studying its oxidative degradation. In the present paper protective effects of bucillamine against the free-radical degradation of HA were investigated. The HA fragments generated were characterized as well. METHODS: To induce free-radical-mediated degradation of high-molar-mass HA under aerobic conditions, we applied Weissberger's oxidative system, comprising biogenic compounds in relevant pathophysiological concentrations, i.e. 100 µM ascorbate plus 1 µM Cu(II). Time-dependent decreases of dynamic viscosity of the HA solutions were recorded by rotational viscometry. Electron donor behaviors of bucillamine were studied by a standard ABTS test method and a chemiluminescence (CL) assay. Ability of incorporation of generated bucillamine thiyl radicals into the biopolymer was verified by Fourier-transform infrared spectroscopy (FT-IR) and size exclusion chromatography with a multi-angle light scattering photometer (SEC-MALS). RESULTS: Decrease of HA viscosity reflected HA degradation. The drug tested was applied in two arrangements: to prevent •OH radical generation (1) and ROO• type radicals propagation (2). Bucillamine, which acted as an efficient •H donor, is also a proper electron donor, as proved by ABTS and CL assays. FT-IR and SEC-MALS methods showed that the drug tested did not incorporate into the biopolymer chains. CONCLUSION: Bucillamine significantly protected high-molar-mass HA against free-radical degradation in vitro, and supposedly this positive action of the drug may be involved in its beneficial effect observed in clinical practice.
Maria Banasova; Vlasta Sasinkova; Raniero Mendichi; Tomas Perecko; Katarina Valachova; Ivo Juranek; Ladislav Soltes
Related Documents :
23524109 - Glycosylation of 'basic' alcohols: methyl 6-(hydroxymethyl)picolinate as a case study.
23038019 - Study on the coupling of acyclic esters with alkenes - the synthesis of 2-(2-hydroxyalk...
22925769 - An improved method for the synthesis of protected glycosyl fluorides from thioglycoside...
23752329 - Synthesis of pentadecaphenylenes, their inclusion properties, and nanostructure formati...
21907269 - Constitutive phenolics of harpephyllum caffrum (anacardiaceae) and their biological eff...
24572999 - Visible light photoredox-catalysed intermolecular radical addition of α-halo amides to...
11140609 - Carbonic anhydrase inhibitors: synthesis of schiff bases of hydroxybenzaldehydes with a...
20183609 - Studies on the glycosylation of pyrrolo[2,3-d] pyrimidines with 1-o-acetyl-2,3,5-tri-o-...
23524109 - Glycosylation of 'basic' alcohols: methyl 6-(hydroxymethyl)picolinate as a case study.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-26
Journal Detail:
Title:  Neuro endocrinology letters     Volume:  33     ISSN:  0172-780X     ISO Abbreviation:  Neuro Endocrinol. Lett.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2013-1-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008373     Medline TA:  Neuro Endocrinol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  151-154     Citation Subset:  -    
Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovakia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Risk of single and combined exposure of birds to non-steroidal anti-inflammatory drugs and lead.
Next Document:  Risk of combined exposure of birds to cyanobacterial biomass containing microcystins, acetylcholines...