Document Detail


Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi.
MedLine Citation:
PMID:  8081236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two glycoinositol phospholipids (GIPL A and GIPL B) have been purified from epimastigotes of Trypanosoma cruzi at the logarithmic phase of growth (2 days). The GIPLs differ mainly in the lipid moiety and are similar to the lipopeptidophosphoglycan (LPPG) previously isolated from epimastigotes at the stationary phase (4-5 days). [3H]-palmitic acid was incorporated into 1-O-hexadecyl-2-O-palmitoylglycerol in GIPL A and into a sphinganine ceramide with palmitic acid and lignoceric acid as the fatty acids in GIPL B. The lipids could be released by incubation with phosphatidylinositol-specific phospholipase C (PI-PLC) or glycosylphosphatidylinositol phospholipase D (GPI-PLD) from rat serum. The oligosaccharides share the common core structure of the glycosylphosphatidylinositol (GPI) membrane anchors. Microheterogeneity was demonstrated, as well as substitution by galactose, which is mainly in the furanose configuration as was previously described for the LPPG. However, methylation analysis indicated that 20% of the galactose is present as terminal pyranose units. In infective trypomastigotes, [3H]-palmitic acid was incorporated into the anchor of the Tc-85 glycoprotein. The lipid cleaved by phospholipase C digestion was identified as 1-O-hexadecylglycerol and the main oligosaccharide has the structure of the conserved core of all GPI anchors. [3H]-palmitic acid-labelled Tc-85 released into the culture medium as membrane vesicles showed 80% resistance to the action of PI-PLC. However, after mild alkaline hydrolysis, part of the radioactivity was released by the enzyme.
Authors:
R M de Lederkremer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]     Volume:  27     ISSN:  0100-879X     ISO Abbreviation:  Braz. J. Med. Biol. Res.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-10-13     Completed Date:  1994-10-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8112917     Medline TA:  Braz J Med Biol Res     Country:  BRAZIL    
Other Details:
Languages:  eng     Pagination:  239-42     Citation Subset:  IM    
Affiliation:
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbohydrate Sequence
Fatty Acids / metabolism
Glycolipids / chemistry*,  metabolism
Glycosylphosphatidylinositols / chemistry*,  metabolism
Molecular Sequence Data
Phosphatidylinositols / chemistry*,  metabolism
Protozoan Proteins / chemistry*,  metabolism
Trypanosoma cruzi / chemistry*,  metabolism
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Glycolipids; 0/Glycosylphosphatidylinositols; 0/Phosphatidylinositols; 0/Protozoan Proteins

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