| Free insulin-like growth factor 1 in the vitreous fluid of diabetic patients with proliferative diabetic retinopathy: a case-control study. | |
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MedLine Citation:
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PMID: 12605576 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the study was to evaluate the vitreous levels of free insulin-like growth factor 1 (IGF-1) in patients with proliferative diabetic retinopathy (PDR). For this, a total of 36 diabetic patients with PDR (group A) and 28 non-diabetic patients (group B) in whom a vitrectomy was performed were compared. Both groups were matched by age, sex and serum-free IGF-1. In a subgroup of diabetic patients (n =21) and non-diabetic patients (n =13), vitreous and serum total IGF-1, IGF-binding protein 1 (IGFBP-1) and IGFBP-3 were also determined. Serum and vitreous levels of free IGF-1, total IGF-1, IGFBP-1 and IGFBP-3 were measured by immunological methods. Vitreal proteins were assessed by a turbidimetric method and adjusted for vitreous haemoglobin. Vitreous levels of free IGF-1 were elevated in group A (median, 0.16 ng/ml; range 0.06-0.57 ng/ml) in comparison with group B (median, 0.12 ng/ml; range 0.06-0.22 ng/ml; P <0.001); however, after adjusting for vitreal proteins, free IGF-1 levels were significantly lower in group A in comparison with group B [0.05 ng/mg (0.01-0.45 ng/mg) versus 0.15 ng/mg (0.07-0.66 ng/mg); P <0.001]. The relatively lower free IGF-1 level observed in group A could not be attributed to differences in the distribution of intravitreous IGFBP-1 and IGFBP-3 in relation to total IGF-1. Notably, the contribution of free IGF-1 to total IGF-1 in vitreous fluid was 10% in group A and 42% in group B; these percentages largely exceed that obtained in serum (<1%). Our results suggest that although there is an enhancement of intravitreous free IGF-1 in diabetic patients due to serum diffusion, a deficit in its intraocular production also exists. In addition, these findings support the concept that intraocular-produced free IGF-1 plays a relevant role in retinal homoeostasis. |
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Authors:
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Rafael Simó; Cristina Hernández; Rosa M Segura; José García-Arumí; Laura Sararols; Rosa Burgos; Ana Cantón; Jordi Mesa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical science (London, England : 1979) Volume: 104 ISSN: 0143-5221 ISO Abbreviation: Clin. Sci. Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-02-27 Completed Date: 2003-05-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7905731 Medline TA: Clin Sci (Lond) Country: England |
Other Details:
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Languages: eng Pagination: 223-30 Citation Subset: IM |
Affiliation:
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Division of Endocrinology, Hospital General Vall d'Hebron, Barcelona 08035, Spain. rsimo@hg.vhebron.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Case-Control Studies Diabetic Retinopathy / metabolism* Female Humans Insulin-Like Growth Factor Binding Protein 1 / analysis Insulin-Like Growth Factor Binding Protein 3 / analysis Insulin-Like Growth Factor I / analysis* Male Middle Aged Research Design Vitreous Body / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Insulin-Like Growth Factor Binding Protein 1; 0/Insulin-Like Growth Factor Binding Protein 3; 67763-96-6/Insulin-Like Growth Factor I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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