Document Detail


Free fatty acids and sterols in human eccrine sweat.
MedLine Citation:
PMID:  2638916     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lipid content of human sweat was determined in thermally induced sweat collected over a Vaseline or silicone barrier placed on the skin (clean sweat) and in sweat scraped from the skin surface without a barrier coating (scraped sweat). Lipids were extracted from concentrated sweat samples into chloroform:methanol and estimated by thin-layer chromatography in conjunction with photodensitometry. Scraped sweat contained 4 to 10 times more lipid than clean sweat and included cholesterol sulphate and ceramides resembling those found in the stratum corneum. In contrast, clean sweat contained only small amounts of free fatty acids and sterol. A marked individual and daily variation in sweat lipid content was also noted. The study indicates the importance of avoiding epidermal contamination when collecting sweat and the usefulness of our sweat collection method.
Authors:
T Takemura; P W Wertz; K Sato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The British journal of dermatology     Volume:  120     ISSN:  0007-0963     ISO Abbreviation:  Br. J. Dermatol.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1990-08-16     Completed Date:  1990-08-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  43-7     Citation Subset:  IM    
Affiliation:
Department of Dermatology, University of Iowa College of Medicine, Iowa City 52242.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Fatty Acids, Nonesterified / analysis*
Humans
Lipids / analysis
Male
Sterols / analysis*
Sweat / analysis*
Grant Support
ID/Acronym/Agency:
AM 25339/AM/NIADDK NIH HHS; AM 27857/AM/NIADDK NIH HHS; AR 32374/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids, Nonesterified; 0/Lipids; 0/Sterols

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