Document Detail


Free fatty acid metabolic profile and biomarkers of isolated post-challenge diabetes and type 2 diabetes mellitus based on GC-MS and multivariate statistical analysis.
MedLine Citation:
PMID:  20846914     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Isolated post-challenge diabetes (IPD, 2h-PG ≥11.1mmol/L and FPG <7.0mmol/L) is often ignored in screening for diabetes by fasting plasma glucose (FPG) levels. The aim of this study was to investigate the metabolic profiles of serum free fatty acids (FFAs) and to identify biomarkers that can be used to distinguish patients with IPD from those with type 2 diabetes mellitus (T2DM) or healthy control individuals. FFA profiles of the subjects were investigated using gas chromatography-mass spectrometry (GC-MS). Principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used for classification and prediction among the three groups. The predictive correct rates were 92.86% for IPD and healthy control individuals and 90.70% for T2DM and healthy control individuals, indicating that PLS-DA could satisfactorily distinguish IPD individuals from healthy controls and those with T2DM. Finally, palmitic acid, stearic acid, oleic acid, linoleic acid and α-linolenic acid were identified as potential biomarkers for distinguishing IPD from healthy control and T2DM individuals. These potential biomarkers might be helpful for diagnosis and characterization of diabetes.
Authors:
Liyan Liu; Ying Li; Chunmei Guan; Kang Li; Cheng Wang; Rennan Feng; Changhao Sun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-16
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  878     ISSN:  1873-376X     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-05     Completed Date:  2011-01-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  2817-25     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Affiliation:
Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, 157 Baojian Road, Nangang District, Harbin 150086, PR China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Analysis of Variance
Biological Markers / blood
Case-Control Studies
Diabetes Mellitus, Type 2 / blood*
Fatty Acids, Nonesterified / blood*
Female
Gas Chromatography-Mass Spectrometry
Humans
Least-Squares Analysis
Linear Models
Male
Middle Aged
Principal Component Analysis
Reproducibility of Results
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Fatty Acids, Nonesterified

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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